Effects Of Sodium Magnesium Fructose Diphosphate On The Intracerebral Hemorrhage

Background and PurposeIntracerebral hemorrhage (ICH) is one of the commonest diseases with high disability rate and mortality in clinical practice. Hematom removal and medical therapy are the main of treatments after ICH up to now. However, many studies have shown that surgery isn't superior to medical therapy. The secondary damage of perihematoma tissue plays an important role in high disability of patients with ICH. So it will be another direction of ICH to seek for safe and effective neuro -protective compounds to alleviate the injured neuron around the hematoma in medical therapy.Studies have shown that FDP and Mg2+ are all capable of protect -ion from cerebral ischemia. As a new chelate salt of FDP and Mg2+, FDPM can protect myocardium and cerebral ischemia efficiently. But no one knows whether it can protect intracerebral hemorrhage, or its protections are superior to those of FDP or Mg2+.MethodsICH was induced in adult rats by stereotaxical injection of bacteri -al collagenase into left caudate nucleus. The effects of FDPM on neurological scale, brain water content, permeability of blood brain barrier (BBB) and pathological change were observed. By NADPH- diaphorase histochemisty, NOS-positive staining cells were studied during ICH, and the effects of FDPM on it were observed. TUNEL was used to detect deoxyribonucleic acid (DNA) fragmentation.TUNEL-positive cells were quantified and the effects of FDPM on it were also studied.Results 1. FDPM (400mg/kg) can significantly decrease neurological scale, reduce brain edema, improve permeability of blood brain barrier and pathological change after ICH. These effects were significant different from those of FDP (400mg/kg) or MgSO4 (30mg/kg). The protective effects of FDPM were dose dependence manner, but FDPM 133mg/kg was no significant protective effect.2. The number of NOS-positive cells increased as early as 2h after onset of ICH, slightly decreased at 6h, increased again at 12h, peaked at 24h-3d and persisted for more than 7days. FDPM can decrease the number of NOS-positive cells to inhibit activity of NOS to decrease synthesis of NO and protect rats from ICH.3. The neural AP was found in ICH. The number of AP cells increased as early as 6h after ICH, peaked at 24h-3d and decreased at 7d. AP cells were distributed mainly in cortex and hippocampus. FDPM can decrease the number of AP cells and protect rats from ICH.In summary, the present study indicated that FDPM can protect ICH significantly, and its effect is more than that of the similar dose of FDP or MgSO4. The mechanisms of FDPM may be including: (1) Improvement of permeability of BBB and reduce brain edema. (2) Decrease the number of NOS-positive cells and inhibition activity of NOS. (3) Inhibition of AP to reduce cell death.