| Objective: Dendritic cells (DCs), known as the strongest professional antigen presenting cells(APC), played crucial roles in the initiating of high-efficacy and specific anti-tumor immune reaction. It was also considered to be the bridge of the mutual action of T cells and tumor cells. But there has been little study on the significance of DCs immunological function on the development of gastric carcinoma up to now. In this study, the relationship between the changes of DCs immunological function and the clinical pathological features of gastric carcinoma were explored. This, therefore, enriched the theory of tumor biotherapy with DCs. Moreover, DCs were evaluated to be the prognostic index of gastric carcinoma in this study.Methods: The samples of peripheral blood and tumor tissue in 30 cases of gastric carcinoma were harvested for the study. The infiltration of tumor infiltrating dendritic cells (TIDC) in gastric carcinoma tissue was determined by immunohistochemistry method. And the expression of the immunological molecules of the peripheral blood DCs surface in patients with gastric carcinoma was investigated by means of flowcytometry. The proliferation ability of the peripheral T cells stimulated by peripheral DCs in patients with gastric carcinoma was determined by 3H-TdR infusions. In addition, the T cell subgroups, NK cell activity and Fas expression of T cells were monitored by flowcytometry.Results:When compared to those in control group, the expressions of HLA-DR, CD80 on the peripheral blood DCs surface in patients with gastric carcinoma1. and the T cells proliferation ability stimulated by DCs decreased obviously. The immunological function of peripheral DCs was significantly correlated with the TNM stage and lymph node metastasis, but not correlated with the site, differentiation and infiltration degree of gastric carcinoma.2. The enhance infiltration degree of TIDC in the tumor tissue of patients with gastric carcinoma decreased significantly along with the progression of the carcinoma and the exhibition of lymph node metastasis. But this was not in accordance with the site, differentiation and infiltration degree of the tumor.3. The peripheral CD3+,CD4+ and NK cell activity and CD4+/CD8+ ratio in patients with gastric carcinoma decreased obviously when compared with those in normal control group. But CD8+ cell increased significantly. These changes were not correlated with the developing site and the differentiation degree of gastric carcinoma, but evidently correlated with the TNM stage, infiltration degree and lymph node metastasis of gastric carcinoma. The peripheral CD4+/CD8+ ratio and NK cell activity were significantly and positively correlated with DCs immune function.4. The ratio of Fas expression of peripheral T cells in patients with gastric carcinoma was much higher than that in normal control group, and which was obviously negatively correlated with the immunological function of peripheral blood DCs.Conclusion:The immunological function of the DCs from the peripheral blood and tumor tissue in patients with gastric carcinoma decreased significantly. This might be one of the important causes leading to the decrease of the immunological function of peripheral blood cells. It might be helpful to the evaluation of the prognosis of patients with gastric carcinoma by determining the immunological function of peripheral DCs and the infiltration degree of TIDC in the gastric carcinoma tissue. The biotherapy of gastric carcinoma withmight be helpful to the improvement of the immunological function of peripheral blood cells, so that the prognosis of patients with gastric carcinoma improved.
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