The Relationship Between The Expression Of P14ARF And P53 And The Efficacy Of E1B--55KD-deleted Adenovirus In Malignant Tumor

To investigate the relationship between the expression status of P14ARF gene protein and mutant -type P53 in human tumor tissue and the efficacy of E1B-55KD E1B-55 KD -deleted adenovirus in malignant tumor, and to explore the mechanism of E1B-55KD deleted adenorvirus gene therapy. Methods: The gene protein of P14ARF and P53 in human tumor tissue were detected in 16 advanced cancer patients treated with E1B-55KD-deleted adenorvirus by immunohistochemistry assay, to analyse the relationship between the expression status of the gene protein of P14ARF and P53 in tumor tissue and the efficacy of E1B-55 KD -deleted adenovirus. Results: The total rate of expression of mutant-type p53 gene and P14ARF gene, was 62.5%(10/16) and 62.5%(10/16), respectively. The total rate of response of 16 patients treated with E1B-55 KD - deleted adenovirus was 37.5%. Six patients retaining wild-type P53 gene and two patients retaining normal P14ARF gene were not evaluable for response, but two in four patients losing P14ARF gene were assessed as response to E1B-55KD -deleted adenovirus. Conclusions: Human cancer cells harbouring mutant-type P53 gene can be killed by E1B-55KD-deleted adenovirus efficiently, but itisn' t only one mechanism. Human tumor cells retained wild-type P53 gene and losing P14ARF genes were efficiently killed by E1B-55KD -deleted adenorvirus. This finding indicates that loss of P14ARF gene is likely another mechanism that human cancer cells retaining wild-type P53 gene can be efficiently killed by E1B-55KD -deleted adenorvirus.