Comparative Study On ERG11 Gene Of Candida Albicans Between Yeast Form And Hyphal Form

Objective:To confirm the suitable hyphal form inducing medium which we have known. To analyze thedifferences on gene sequences between yeast form and hyphal form of Candia albicans, ERG 11gene encoding the target enzyme of fluconazole and its sequences were compared in this study. The evidence for selecting the form of Candiada albicans in medical mycology would be provided.Methods:A series of 16 strains Candida albicans from the same body (CA-1, CA-2, …A-17, but without CA-10 ) were induced to form hyphae or/and pseudo-hyphae in RPMI1640 medium with 10% calf serum at 37癈 for seven days bytransferring twelve times. The yeast form and hyphal form cells were counted and theI ratio of hyphal form cells was caculated. Seven sets of primers were designed toamplify the ERG 11 gene. DNA sequences of these fragments were compared through Single-strand conformation polymorphism (SSCP) analysis and DNA sepquencing.Results:1. The ratio of hyphal form cells of all the strains was more than 99% afer beingcultured for seven days by transferring twelve times in RPMI1640 medium with 10% calf serum at 37℃.2. The ERG 11 gene of all strains had been amplified triumphantly.3. There were some differences on ERG11 DNA sequences between yeast forrh and hyphal form of Candida albicans.Conclusion:1. RPMI1640 medium with 10% calf serum to induce hyphal form at 37℃ forseven days was thought to be the favorable condition for Candida albicans hyphal form indution.2. There were some differences on gene sequences between yeast form and hyphal form of Candida albicans.3. Hyphal form of Candida albicans was thought to be the appropriate object of medical mycology.