Preparation, Characterization And Biocompatibility Evaluation Of Novel Biodegradable Crosslinked NVP Scaffold Materials

The biodegradable crosslinking agents were synthesized by polycondensation reaction of lactide using glycerol as initiator and stannous octoate as catalyst, followed by functionalization of the hydroxyl end groups with acryloyl chloride in the presence of triethyl amine in this research. The polymers were characterized using GPC, FTIR and 'H-NMR ananlysis and the results were consistent with the expected. A series of biodegradable crosslinked NVP scaffold materials were prepared by radical polymerization of the biodegradable crosslinking agents and NVP using azoisobutyronitrile (AIBN) as initiator. The different characterizations of materials, ratio of water absorption, contact angle and degradation, were also detailedly elucidated along with different qualities and different molecule weight of crosslinking agents. Finally a kind of porous scaffold material was made by means of salt out. The porous scaffold material was researched by scaning electron microscope (SEM). Cell culturing and planting in vitreous body were used to evaluate their biocompatibilities.GPC was used to study the relationship between molecule weights and the radio of raw materials in the synthesization of crosslinking agents. The result showed, molecule weights of crosslinking agents would be controlled by changing the ratio of glycerol and lactide. Through FTIR and 'H-NMR ananlysis hydrochloric acid - doffed reaction was easy to produce multi-vinyl biodegradable crosslinking agents. With the initiation of AIBN, a simple method of simultaneous membrane formation and reaction of the mixture of crosslinking agent and NVP was used to get a series of membranes. It showed that, ratio of water absorption would increase along with the decrease of the quantity of crosslinking agent and the molecule weight of crosslinking agents. As for contact angle, itincreased at the same time of increasing the ratio of crosslinking agent. But the effect of molecule weights of crosslinking agent on contact angle was not in evidence. In the degradation process, the mass-loss rate of the materials showed specific original kinetic in the early duration, but it increased dramatically at last. Through the investigation of cell culturing and planting in vitreous body, the biocompatibility of the materials was quiet good, which brought it a bright future in the tissue engineering fields.