| ObjectiveOvarian cancer is the malignant tumor which seriously imperil health of women, the death rate of which hold primacy in gynecologic tumor. Chemiotherapy is important in treatment of ovarian cancer, but because of generation of resistance to chemiotherapy, population survival rate of ovarian cancer patiens is not optimizing. Cyto0biology considers that immortalisation of cell is necessary in process of cell carcinomatous changing. Telomerase can extend telomere, when it is activised abnormally. It is origin of cell immortalisation.The experiment detected telomerase activity of sensitive and resistant ovarian cancer cell strains to cispiatin before and after treated by cispiatin, and inhibited telomerase activity of different ovarian cancer cell strains, to study effect of telomerase inhibitors to ovarian cancer cell strains with different sensitivity to cispiatin, try to find a new method to avoid production of resistance to routin chemiotherapy.Materials and Methods1. Cell strainThe sensitive human ovarian epithelial cancer cell strain to platinum compounds ( A2780) and the resistant human ovarian epithelialcancer cell strain (CAOV3 and OVCAR3) were used.2. Cell cultivationCell strains were cultivated in RPMI-1640 culture medium containing 10% fetal bovine serum, and 0. 1ml cell culture medium, which contained 2×104/ml cells, was added in 96 pore culture plate to be cultured.3. Drugs: CDDP, AZT, ATRA(1) Three ceD strains were cultured in 1×10-5M,3×10-5M,5× 10-5M,8×10-5M,10×10-5M CDDP for 24 hours.(2) Three cell strains were cultured in 3×10-4M,6×10-4M,10 ×10-4M or 4×10-5M,6×10-5M,8×10-5M AZT for 4 days.(3) Three cell strains were cultured in 10-8M,10-7M,10-6 M ATRA for 6 days.4. Detection method of cell proliferation inhibition effect and te-lomerase activityCell proliferation inhibition effect of drugs was detected in MTT. Rate of cell proliferation inhibition was calculated with the formula, (1-ODdetected/ODcontrol)×100%. Every experiment was repeated 4 times.Telomerase activity was detected in TRAP-ELISA. It was indicated in total product generated, TPG:TPG={[(TP-B)/TI]/[(R-B)/RI]}×100.TP,B,TI,R and RI represented respectively absorbency of detected sample, splitting buffer, interior control of detected sample, standard control and interior control of standard control. Every sample was repeatedly detected 4 times.5. Statistic calculationData was expressed in mean value+standard deviation (x+s).Difference of drugs effect to different cell strains was verified with a-nalysis of variance. Dosage - effect relation was verified with linear repression. P<0. 05 illuminated that difference was statistically significative.Results1. Telomerase activity was not significative different between sensitive cell strain (A3780) and resistant cell strains (CAOV3 and OVCAR3), when no treatment factor was imposed. Telomerase of sensitive cell strain (A2780) decreased significatively, while telomerase activity of resistant cell strains ( CAOV3 and OVCAR3 ) didn 1 change significatively, after cell was treated with 5×10-5 M CDDP.2. 3 ×10-4M,6× 10-4M,10× 10-4M AZT can inhibit obviously proliferation and telomerase activity of A2780 and CAOV3 with dosage- effect relation. Effect of the drug had not significative difference between sensitive cell strain ( A2780 ) and resistant cell strain (CAOV3). 4×10-5M,6×10-5M,8×10-5 M AZT can inhibit obviously proliferation, but AZT didnt affect telomerase of OVCAR3 significatively in the concentration.3. 10-7M,10-6M ATRA can inhibit proliferation and telomerase activity of A2780 and OVCAR3 observably. And effect of it to sensitive cell strain (A2780) and resistant cell strain (OVCAR3) had not significative difference.Conclusion1. Proliferation inhibition effect of CDDP to sensitive and resist-ant cell strains was significatively different.2. Telomerase activity was not distinctly different between sensitive and resistant cell strains before CDDP...
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