Study On PTEN Gene Expression In Epithelial Ovarian Carcinoma

Objectives To investigate the expression of PTEN (phosphatase and tension homology deleted on chromosome ten) mRNA in epithelial ovarian carcinoma as well as to elucidate its clinical significance Methods The expression of PTEN mRNA in 49 cases of epithelial ovarian carcinomas, 15 benign ovarian tumors and 20 normal ovarian tissues was detected by reverse transcription -polymerase chain reaction: the relative mean expression of PTEN mRNA in these same samples was detected by semi-quantitative RT-PCR. Results Of the 49epithelial ovarian carcinomas, the respectively positive expression of PTEN mRNA was exon 5-8 38(77.55%) exon 5-9 37(75.5%) exonl-8 35(71.43%) exon 1-9 35(71.43%). However, the positive expression of PTEN mRNA was found in all of the 15 benign ovarian tumors and 20 normal ovarian tissues: Of the 49 epithelial ovarian carcinomas, the relative mean expression of PTEN mRNA to GAPDH was exon 5-8 0.83 0.17, exon 1-8 0.78 0.14. Of the 20 normal ovarian tissues ,the relative mean expression was exon 5-8 1.42 0.29, exonl-8 1.37 0.24. The expression of PTEN mRNA did not show correlation with such factors as age, histological type, but had much to do with the clinical staging and histological grading. Conelus i on The expression level of PTEN mRNA in epithelial ovarian carcinomas was sharply reduced compared to normal ovarian tissues, which suggests that PTEN mRNA inactivation plays an important role in the genesis and development of epithelial ovarian carcinoma. The expression of PTEN mRNA was expected to be the diadynamic criteria of epithelial ovarian carcinoma .