Cytology Of Oral Carcinoma After Microwave Tissue Coagulation

Objective: Our experiment observed specimens by optical microscope and transmission election microscope after microwave tissue coagulation of oral carcinoma. We discussed the cytological changes of cancer tissue and the relationship among MTC, apoptosis and immunoen- hancement. We compared MTC with tradional surgery and proved its practicable and availability. In short, MTC was an advanced mathod in complex treatment on oral carcinoma. Material and methods: 32 patients, hospitalized in the Fourth Hospital of Hebei Provice (from Surp. 2000 to Surp. 2002) ,participated in this study. The lesions included were as follows:oral and maxillofacial squamous cell carcinoma (Oscc) 28 cases (well-differentiated 17 cases), salivary adenoid cystic carcinoma(Acc) 4 cases.Among them the generative site was respectively bucca(10 cases), lingua(8 cases), labia(8 cases), gingival (4 cases),carcinoma cutaneum faciale (2 cases). According to the standard of UICC, 7 patients in stage I, 6 in stage II, 7 in stage III and 5 in stage IV. All the patients assisted with chemotherapy and/or lymphadenectomy of suprahyoid region. After MTC we extracted tissues for pathological examination immediatelyand morphologic observation by transmission electron microscope. 32 patients, selected randomly from 50, accepted tradional surgery were studied as controls. Their ages, sexes, pathomorphological classifications, clinical stages and pathogenic regions were similar to the experimenters. All the patients were follow-up surveyed from 6 months to 2 years. The therapentic effects of the two groups were observed and compared. Result: 1. Postoperative examination of optical microscope: The pathology result of 5 patients among experiment group have no obvious changes compared to previous, so they accepted secondary operation or microwave tissue coagulation . The others pathology reports were degenerative and necrotic tissue, inflammatory granulation, exudative necrosis, hyperplasia or atypical hyperplasia. Control group had no obvious changes after surgery. There was significance difference micropathology between experiment group and control group(P<0.05). 2. Postoperative transmission electron microscope observation: Ecperimental group showed: â‘  Tumors turned into degeneratic and necrotic tissue. â‘¡ Apoptosis was observed beside focus: part nuclear membrane sticked up; cytolipin concentrated, nulear heterochromatin aggregated on the nuclear membrane , local eminence like halfmoon; karyopyknosis, karyoclasis and apoptosis body formed. Control group had no increased apoptosis. The differencebetween the two group is significant (P<0.05). â‘¢ Besides apoptosis lots of mastocyte, contained special hypertrophic granule, were also observed. Control group had little mastocyte, which may have something with none-heated of microwave. After statistical analasis, we found the significance difference between the two groups on survival rate , neoplastic recurrence and cancerometastasis in 2 years. That is, MTC has the same effects as the traditional surgery. But MTC can save oral faction, facies and improve survival viability, which is more advanced than the old method(P<0.05).Conclusions: Microwave tissue coagulation can obliterate the primary focus of oral carcinoma favorablely. It control the local recurrence of tumor through evoking apoptosis and enhancing immunity. Compared to the traditional surgery, microwave tissue coagulation has characteristics of slight trauma and side effect, high quality of life. To the oral and maxillofacial malignancy patient, specially the recurrencer MTC is a effective method.