A Study On The Effect Of Different Catheter Materials Against Formation Of Pesudomonas Aeruginosa Biofilm

Objective: To investigate the effect of different catheter materials against formation of P aeruginosa biofilm; To learn the eradicative efficacy of various treatment to different growth period of biofilm.Methods: P.aeruginosa biofilms were prepared with four kinds of catheter, which were made of latex, siliconized-latex, pure silicone and rubber ,incubated with P.aeruginosa for 7 days, and the viable bacteria counts of biofilm were performed by serially diluted method. In addition, three ways including washing, washing and vortex agitation, washing and ultrasound were employed to treat P. aeruginosa biofilm in different growth period.Results: The viable bacterial counts of biofilm adhered to latex and siliconized latex, were 1.91x107cFu/cm3andl.20 X 107cFu/cm3, respectively ,compared with 2.7x105cFu/cm3of biofilm adhered to pure silicone, The counts of biofilm adhered to rubber were slightly higher than pure silicone ,which is 6.5x106cFu/cm3.Statistical difference consisted in the counts of biofilm adhered to four kinds of materials.The eradicative efficacy of various treatment showed, that threeways had similar efficacy of eradicate the young P. Aeruginosa biofilm, but washing was difficult to eradicated the mature biofilm from catheter ,compared with the strongest eradication of wash plus ultrasound .Conclusion: There was a certain relationship between biomaterial and formation of P. aeruginosa, catheters made of latex and siliconized latex were easily adhered to bacteria and formation of P. aeruginosa, compared with pure silicone and rubber; washing was effective methods to eradicate young biofilm adhered to catheters.