Study On The Plasma Levels Of And The Correlations Between ADM And CAMP,NO,NOS In Patients With Congestive Heart Failure

Background and Objective:It has been proven in recent study that cardiovascular system is not only a circulating tissue, but also an important endocrine function, including cardiomyocyte, vasculaur endothelial cell(VEC) and vascular smooth muscle cell(VSMC) which can produce many kinds of bioactive molecule,affecting congestive heart failure's occurring,developing and lapse from different cellar conduction access. Plasma levels of ADM,cAMP,NO and NOS were determined in patients with congestive heart failure. Materials Mcthds:1. Cardiothoracic ratio,left ventricular distolic diamension, left ventricular eject fraction and E/A were measured in 45 patients with congestive heart failure(CHF) and 20 controls.2. Blood samples were collected,centrifuged under low temperature.Blood plasmas were stored in refrigeratory of -70癈.3. Plasma levels of ADM, cAMP were measured by specific radioimmunoassay. NO were measured by nitric acid revivification enzyme.NOS were measured by chemical method.Results:Camparing clinic symptoms of CHF groups with that of control:1. There were no significant differences in sex and age between CHF groups and controls. Left ventricular ejection fraction significantly decreased in patients of class III and IV as compared with the controls(P<0.05), but there were not significant between the class II and controls. Cardiothoracic ratios of patients of class III and IV were significantly higher than the controls(P<0.05).Left ventricur end-diastolic diamention significant increased in patients of class III, IV as compased with class II and controls;Left ventricular diastolic function(E/A) significantly decreased in all patients as compared withthe controls(P<0.05).2. Camparing plasma ADM concentration of CHF groups of before treatment and after treatment with that of controls:Plasma ADM concentrations significantly increased in class IK III as being compared with controls.They reached the top in class III.They were significantly different between class II and III(P<0.05).They significantly decreased in class IV as being campared with controls,class II and III(P<0.05).After treatment,they significantly decreased in class II and HI(P<0.05),but they increased in class IV (P>0.05) .3. Camparing plasma cAMP concentration of CHF groups of before treatment and after treatment with that of controls:Plasma cAMP levels were significantly higher in class 11^ III and IV than that of controls(P<0.05). They reached the top in class III. They were significantly deferent as comparing class III with class II and IV (PO.05) .Levels of class IV were lower than class II and III ,but higher than controls(P<0.05). After treatment, they significantly decreased in all patients as compared with before treatment (P<0.05)..4. Camparing plasma NO concentration of CHF groups of before treatment and after treatment with that of controls:Plasma NO levels in class II and III were significantly higher than controls(P<0.05). Levels in class III were significantly higher than in class II(P<0.05).Levels in class IV were lower than in class II and III (P<0.05) , but not significantly different with controls (P>0.05) . After treatment, they significantly decreased in all patients as compared with before treatment (PO.05).5. Camparing plasma NOS concentration of CHF groups before treatment with that of controls:Plasma NOS levels in class II and III were significantly higher than controls(P<0.05).Levels in class IV were significantly lower than in class II and III, but not significantly different with controls (P>0.05 ) .6. There was positive correlation between ADM and cAMP in CHFpatients (r=0.726 p<0.01) .7. There was positive correlation between ADM and NO in CHF patients (r=0.650 p<0.Ol) .8. There was positive correlation between ADM and NOS in CHFpatients (r= 0.567 p<0.01) . Conclusion:1. Plasma levels of ADM, cAMP, NO and NOS were correlative closely with the degree of heart failure. They...