A Preliminary Investigation On The Synchronization Of Hepatocellullar Carcinoma Cells And Its Adhesive Properties With Extracellular Matrix

Malignant tumors are a kind of disease that seriously imperil the health as well as lives of human beings. Characteristics of malignancies include uncontrolled proliferation, invasion and metastasis there have been great improvements in the researches of tumor invasion and metastasis with the fast development of cellular biology, molecular biology and immunology. However, there are not enough studies on the cell rheological properties, which have intimate relations with tumor invasion and metastasis. Study and comparison are carried out from different angles. Such researches may be theoretically as well as practically important for an understanding of tumor cell invasion and metastasis.The following are the main results of the present study.(1) Synchronous G1 phase cells cells were got by thymine-2-deoxy- ribo nucleoside and colchicine blocking in succession, synchronous S phase cells were achieved by double thymine-2-deoxyribonucleoside blockage method. The synchronization results evaluated by flow cytometer showed that it could meet the requirements of the experiments nicely.(2) The mircropipette aspiration technology was used to study the adhesive properties of CBRH7919 cells to collagen I. The experiments shows that the adhesion forces are concentration dependent. On the other hand, the adhesion forces increases with the continunce of the time. Where the rates of increase are limited in the later time. The G1 phase cells have a strong adhesion forces than the S phase in the viewpoint of cell cycle. This reflects the higher potential of metastasis of G1 phase.(3) The experiments on the adhesive properties of HCC to collagen IV are also carried out. The results show that the adhesion forces are concentration and time dependent. The adhesion forces of HCC cells are drastically unstable in the preliminary state. The S phase cells has a lower adhesion forces than the G1 phrase under the synchronized disposition. This may reflects the active biochemical reactions in the cells in G1 phase.(4) Some analyzations and comparisons were carried out which are the researching results of adhesion properties between different cancer cells and the extracellular matrix with the aid of micropipette aspiration technology. The resultsshows that the adhesion forces are concentration dependent despite the variation of cell line. This may reflects the important of collagen for the adhesion of cancer cells. It provides not only the defense system but also the prop of the metastasis.