Clinical And Immunohistochemical Analysis Of Primary Cholangiocarcinoma. Accompanying With Hepatolithiasis

The etiology of cholangiocarcinoma is not known. A mass of clinical data manifested from homeland and other countries that calculus of bile duct is one of the induced factors. In recent years, increasing cases of contained-stone cholangiocarcinoma was observed in hospital, so that it is essential to know the law of diagnosis and therapy then serve to clinical.This article include two parts. The first part was retrospectively to analyse clinic datas came from patients treated in our hospital between 1991 to 2000 and sum up their experiences.The second part was the collection of samples of paraffin wax from these cases and Immunohistochemical methods were used to studied the expression of P53,Bcl-2 and C-met protein in 30 case of cholangiocarcinoma .In all 45 cases diagnosed chcholangiocarcinoma patients , the history of hepatolithiasis over 10 years were 24 cases by 53.3% and have over and over cholangeitis at the same time, indicated that the dangers were increasing to take place chcholangiocarcinoma in hepatolithiasis patients and hepatolithiasis is related to chcholangio- carcinoma .When hepatolithiasis and chcholangiocarcinoma coexisted, clinic diagnosis of chcholangiocarcinoma was very difficulty .Through reviewed datas from definitive diagnostic patients before operation indicated that combination of CT and colored ultrasound obviously improve clinic diagnosis rates and guidance treatment projects.The hepatolithiasis distribution was limited as locals and permeations. The second pattern with cholangiocarcinoma may be therapy through localliver lobe resection.The third pattern with cholangiocarcinoma maybe also cut out .To sum up,the therapeutic effect of hepatolithiasis accompanying with cholangio-carcinoma was poor.so hepatolithiasis shoud be early radically therap.Cholangiocarcinoma is a genic disease . We selected p53 and c-met and bcl-2 to investigation which were little researched about cholangiocarcinoma in home and other land but were certified that they play a important role in primary hepatocellular cancer and other neoplasm. To know about its expression in cholangiocacinoma and assay their relation between three gene and evolution of neoplasm,so that to use to clinic diagnosis and therapy.Results of Immunohistochemical methods indicated P53,Bcl-2 and C-met in contained-stone cholangiocarcinoma had higher expression in particular Bcl-2 and C-met ,They may have obviously relationship with contained-stone cholangiocarcinoma but did not relate with differrentiation of cholangio- carcinoma. P53,Bcl-2 and C-met expression in accompanying with cholangiocarcinoma deserve to explore.