Research Of Dynamic Of Calcium In The Hippocampal Neuronal Culture Model And Long-term Clinical Observation Of Topiramate In The Treatment Of Epilepsy

Section I Dynamic of calcium in the hippocampal neuronal culture model of epilepsyObjective Earlier studies have indicated that epilepsy occur with the nerve cell calcium overloading, the abnormal calcium ions inflow can bring about a series pathophysiology changes which lead the nerve cell injured and long-term plasticity changed. But there is few research about the dynamics of intracellular calcium ion C[Ca2+]j) .Our research is to observe the intracellular calcium ion spatio-temporal and dynamic changes in the hippocampal neuronal cultured model of epilepsy induced by low magnesium ion medium, and to explore the relationship between calcium ion and epilepsy.Method Appling both laser scanning confocal microscope and cell culture tecchniques to timely observe the changes of [Ca2+]; in the hippocampal neuronal culture model of epilepsy, and the influence with [Ca2+]j by NMDA receptor-gated channels retarder and non-NMDA receptor-gated channels retarder.Result After the hippocampal nerve cell broken into epileptiform discharges, [Ca2+]i rapidly ascended to 620?0 nM, NMDA acceptor retarder (MK-801,10uM) and non-NMDA acceptor retarder (NBQX,10uM) reduced [Ca2+]i ascendance. Recover of the elevated [Ca2+]i is obviously delay, after 90min and ISOmin epileptiform discharges, it took 114.8±5.2 and 135±22.7 (p<0.05) respectively. Conclution1. After the hippocampal nerve cell epileptiform discharges for a long-term, Recover of the elevated [Ca2+]{ is obviously delay.2. Receptor-operated calcium channel may participate the process of [Ca2+]j influx to hippocampal nerve cell.