| This paper introduced the morphological character, geographic distribution, chemical constitutes and pharmaceutical function of Forsythia suspensa. It also studied the anti-oxidative effect and the effect of protecting liver of Forsythia suspensa leaves' tea and evaluated the safety of its usage.Forsythia suspensa vahl. belongs to Oleaceae's defoliate shrub. It is two to four meters high. Its branch is unfold and the flower is yellow. Its fruits are tip and egg-shaped. It mainly grows in hilly area of Henan province, Shanxi province, Shaanxi province, Sichuan province and so on. It mainly contains terpenes, phenylethanol, flavonoid, lignan, alcohol, ester, aldehyde, ketone, volatice oil and so forth Forsythin forsythigenol, oleanolid acid, rutinoside, lignan are the main effective compositions among them.Traditionly the fruit of Forsythia suspensa is regarded as a kind of drug. Its effects are extensive, such as antiviral activity, antibacterial action, cardiotonic action, diuresis, protecting liver, decreasing blood pressure and so on. The folk investigation shows Forsythia suspensa leaves have the effects of eliminating constipation, preventive common cold, reducing blood fat and so on. Some studies showed the content of effective compositions in Forsythia suspensa leaves is higher than in the fruits Forsythia suspensa leaves were made into tea as nutritious drink. The results of analizing its microelement show that Forsythia suspense leaves contain abundant elements which are necessary to body, such as Mg, Zn, Se. But the pharmaceutial function of Forsythia suspensa leaves has no report.The safety of usage about Forsythia suspensa leaves' tea was studied by means of the maximum tolerated dose of mice; In vitro anti-oxidative studies the followingindexes were measured:the elimination of O2- was determined through pyrogallolautooxidation, the elimination of ?OH was determined through FeSO4+H2O2 producing · OH, the function of protecting liver mitochondrion was studied by means of Fe2++Vc inducing the swelling of mitochondrion, the effects of FSE on liver, brain, heart, spleen, kidney were studied by means of FeSO4+H2O2 inducing oxidative lesion. In anti-oxidation and protecting liver studies, we used ICR mice as the experimental animals FSE was fed to mice for 20 days. Mice were injected alloxan (150mg/kg) into abdominal cavity to induce oxidative lesion. The anti-oxidative indexes were measured to confirmthe anti-oxidative effects of FSE. The results of experiments show:1. The maximum tolerated dose of mice to FSE is 62.5g/kg, as 750 times as daily usage of man (5g). This shows that the toxicity of FSE is tiny, so it can be used safely.2. In vitro FSE have the · OH and O2- scavenging activities. It can inhibit thegeneration of MDA, the hemolysis of red blood cells and the swelling of mitochondrion in vitro.3. FSE has evident effects on liver injury and oxidative lesion which caused by alloxan. It can inhibit obviously the abnormal increase of SOD, POD, MDA in liver, brain, heart, musculus quadriceps femoris tissue, red blood cell and ALT, AST, AKP in blood serum induced by oxidative lesion,the fall of liver index and spleen index.4. FSE can reduce blood sugar induced by alloxan and increase the level of liver glycogen and muscle glycogen.These suggest FSE maybe can improve mice physical performance.5. FSE can inhibit the growth of adult mice weight. This suggests FSE maybe has the function of losing weight and reducing blood fat. This needs further study.6. Three dosages of FSE all have the effects on oxidative lesion mice, and the effects have little disparity. This shows the dosages of 0.5g/kg and1.5gAg can be as reference dosage.This study shows FSE has the effects of antioxidation and protecting liver. Its toxicity is tiny, so it can be used safely. These results can provide the theoretical basis on Forsythia suspensa leaves' pharmacologic research and extend Forsythia suspensa leaves' exploration. |
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