Study Of Elution Methods Of Thin-Alyer Chromatography And The Application In The Pharmaceutical Analysis

Capillary force is the mainly driving force of the thin-layer chromatography (TLC). For this reason, the on-line detection that used in the column chromatography is difficultly realized in the classical TLC. In view of this weakness, we started our work to solve this problem.The descending development was studied firstly. We hoped to reach the aim with the help of the gravity. A new solvent-delivering model of descending development was developed. That changed the conventional fashion delivering the solvent by using filter paper. Many capillary-drafting instrument were designed in order to realize on-line detection, but they all cannot meet the need. The results of the experiments showed that the capillary force but not the gravity mainly delivers the solvent. So, without other outer force, it is difficult to reach the aim of on-line detection. However, with the help of a simple and effective collector and a negative pressure system, the method of separation coupling elution was developed successfully. Based on this principle, separation coupling elution in horizontal development and in-situ elution was developed.ABSTRACTThe efficient of these elution models is high. That reduces the complex operation procedure of scraping the spots off the silica layer. This method was applied in the preparation of pure substances of traditional Chinese medicine. Three kind of anthraquinone were separated and got from rhubarb. The purities of these three substances were better than those that were got by column chromatography.The high elution efficient of separation coupling elution and in-situ elution makes them reliable operation. The content of three anthraquinone in cassia were measured by these methods. That provides chromatographer with a new selection to analysis and deal with traditional Chinese medicine.The analysis of phosphalipids is a difficult problem, for it is harmful to the column of high performance liquid chromatography (HPLC), and the result that got by the TLC scanner were always instable. The in-situ elution and molybdenum blue reaction was used to measure the content of the phosphacholine (PC) in the egg yolk phosphalipids. The result is satisfied.Studied the methods of separation and elution systematically, a few of misunderstandings and fantasy were cleared up. The theory of the separation of the TLC was deeply recognized. The method that on-line elution in descending and horizontal development and the in-situ elution provide many new operations for the chromatographer.