Studies On Thrombolytic Effects And Mechanism Of Nattokinase

Heart and brain vessel disease is an important disease which do harm to human health. At present, urgent affair is to look for a kind of cheaply, innocuously, effciently thrombolytic medicine. Natto which is a typical soybean food eaten in Japan was used to preventing and treating heart and brain vessel disease previously. Since the nattokinase(NK) was discovered by Sumi in Japan, the Natto have become an important food in the world. So the functional factor - nattokinase which has a strong fibrinolytic activity was researched deeply. This paper included several experiments below: first, studies on fibrinolytic activity of NK; second, thrombolytic experiment of NK in vivo; third, thrombolytic mechanism of NK; forth, safety evaluation of NK. Results: first experiment showed that high, middle, low dosage NK could decrease significantly dissolve time of euglobulin in plasma, and reduce significantly the content of fibrinogen in plama and increase the content of fibrin decompose product. The first experiment indicated that NK has a strong fibrinolytic activity, and it has more fibrinolytic activity than Urokinase(UK) at the same dosage. The second experiment showed that NK could prolong thrombus forming time in blood vessel remarkedly, and could lessen the length and weight of thrombus and promote the ratio of recirculation of the femoral artery significantly, decrease the weight of thrombus in lung. This experiment indicated that NK has a strong thrombolytic action in vivo, and its potency was superior than UK at the same dosage. The third experiment showed that NK could decrease significantly the content and activity of t-PA, but NK hadnt obvious effects on contents of PAI-1. This experiment indicated that thrombolytic activity of NK was obtained by activating t-PA The last experiment showed that NK has innocuity, and no side-effect.