Protective Effect Of Diethylenetriamine Induce Delayed Preconditioning Response On Ischemic Cold-stored Heart During Cardiac Transplantation

Objective The goal of this experiment is to study protective effect of Diethylenetriamine induce delayed preconditioning response (DPR) on Ischemic cold-stored heart during cardiac transplantation and mechanisms of its regulation in myocardial protection. Methods Forty-eight rabbits were divided into two groups randomly. Groupl was accepter group.Group2 was divided into three groups randomly. In group A, the pretreatment of rabbits were given Diethylenetriamine (DETA, 0. lmg/kg/15min, 4times)24 hours before the heart was arrested. In group B, pretreatment with the NF- K B inhibitor diethyldithiocarbamate (DDTC, 150mg/kg 15 minutes before DETA preconditioning). Group C served as controls. The isolated rabbit hearts were arrested with and stored in KH solution at 4癈 for 4 hours before transplantation. Function, enzyme, metabolite and substructure of hearts were observed after transplantation. Results DETA induce DPR provide a relative degree of cardioprotection demonstrated by significantly better preservation of left ventricular compliance, reduced leakage of creatine kinase (CK), LDH, reduce generation of LD reactive oxygen species (ROS), MDA, improve activation of Na+-K+-ATP-enzyme, and smaller degree of subceller destruction. compared with controls (p<0. 05) .These beneficial effects were abolished by DDTCpretreatment. Conclusions These data support the concept that the cardioprotective effects of DPR of ischemic preconditioning can be duplicated by DETA and suggest that activation of NF-B could be an effective means of improving the preservation of globally ischemic cold-stored hearts, as occurs during cardiac transplantation.