The Basic Research Of Immunosenescence Inducing By Oxidative Stress

Objiective: To observe the variation of 8-oxo-dGTPase expression and telomere length of human peripheral blood lymphocytes caused by oxidative stress, and to evaluate the effect of oxidative stress in telomere shortening, to study the possible mechanism of immunosenescence inducing by oxidative stress.Methods: Human lymphoid cells were cultured in vitro and divided into three groups: oxidative, anti-oxidative using Vit.C and normal control group. RT-PCR were applied to study the mRNA expression of 8-oxo-dGTPase. Southern hybridizing were applied to determine telomere length.Results: (1) The expression of 8-oxo-dGTPase was increased significantly in lymphocytes damaged by H2O2; and there was no significant difference between anti-oxidative and normal control group. (2) Telomere length were shorten with the change of time .and both three groups had significant difference (p<0.01).Conclusions: (l)The expression of 8-oxo-dGTPase was increased significantly with increasing levels of DNA damage, and pre-treatment ofcells with Vit.C inhibits the increased expression of 8-oxo-dGTPase induced by oxidative stress. (2)Pre-treatment of cells with Vit.C can slow the rate of telomere shortening. (3) Oxidative stress takes some reaction on telomere shortening.