| Purpose: pancreatic carcinoma is a common malignant tumor of digestive system, Pancreatic cancer is an extremely aggressive neoplasm whose incidence equals its death rate. Pancreatic cancer is the fifth leading cause of cancer deaths. Moreover, 5 - year post - operation survival for patients with organ - confined disease is only 20% , and in the majority of cases, in which the disease when diagnosed has already spread past the pancreas, survival is only 4% . Despite intensive analysis, the genetic changes that mediate pancreatic cancer development remain unresolved. And effective therapies for diminishing the morbidity associated with this disease are very importany. Recent data suggest that apoptosis has closed relationship to the development and progression of tumor. Apoptosis is a idiopathetic process via a Caspase reaction in cytoplasm and mitochondrion and make the cell die. Programmed cell death via the Fas receptor/Fas Ligand plays a major role in tumor escape and elimination mechanisms. There are many studies on the mechanicsms and roles of Bcl - 2, FasL in apoptosis. The recent study show that Bcl -2 is a protein which could inhibit apoptosis, inhibited the cascade activation of Caspase kinase. In this manner, the ratio of inhibitors to activators in a cell may determine the propensity of the cell to undergo apoptosis 0 Previous studies haveshown that various cancer cells express FasL and kill lymphoid cells by Fas - mediated apoptosis. That sssociated with the increased apop-tosis of tumor - infiltraing lymphocytes, thereby contributing to the immune privilege of the tumor, the tumor survival. Then we conduct the exprement in order to explore correlation of the expression of Bcl - 2, FasL protein in pancreatic carcinomas and their adjacent tissues.We analysis the relations and significance of Bcl -2,FasL protein and clinical feature such as sex, age, clinical staging and differentiation of tumor of pancreatic cancer patientsMethodsWe used immunohistochemical method to examine formalin -fixed paraffinembedded samplse of 41 pancreatic carcinomas , 26 nommalignant peri - tumoral dssue and 10 normal pancreatic tissue for overexpression of the Bcl - 2 FasL protein.ResultsWe determin the positive cell according to the distribution of staining cell and the staining intensity in staining section. Specific nuclear staining for the Bcl - 2, FasL protein was identified in 28 and 26 out of 41 carcinomas ,11 and 9 out of 26 peri -tumoral tissues. 3 of 10 nomor tissue express Bcl -2. 3 out of 10 nomor pancreatic tissue express Bcl -2 . We find the positive rate of Bcl -2, FasL was significantly higher in cancer tissues than adjacent tissues ( P < 0. 05 ) use statistic analysis. The expressions of Bcl - 2 and FasL in pancreatic carcinnomas have no relation( R =0. 04). The positive rate of Bcl -2 , FasL has no significant in adjacent tissue and nomor pantreatic tissue. The positive expression of Bcl - 2 protein was significantly higher in clinical stage of I , II and high differentiation than III, IV and low differentiation(P <0. 05) ,and has no relation with sex,age (P >0. 05), The positive expression of FasL protein has no relation with sex, age, clinical stage, differentiation (P >0. 05).Conclusion1. The positive rate of Bcl - 2, FasL was significantly higher in cancer tissues than adjacent tissues.2. The positive rate of Bcl - 2, FasL has no significant in adjacent tissue and nomor pantreatic tissue.3. The expressions of Bcl - 2 and FasL in pancreatic carcinnomas have no relation( R = 0.04).4. The positive expression of Bcl - 2 protein was significantly higher in clinical stage of I, II and high differentiation than III,IV and low differentiation, and has no relation with sex, age. The positive expression of FasL protein has no relation with sex, age, clinical stage ,differentiation. Bcl -2 FasL protein is involved in the carcinogenesis if pancreatic carcinomas, and has relationship with the development and progressive of pancreatic carcinoma. The expression of...
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