| Its confirmed by the nearly 20 years'studies, the oxidative damage enhances in ESRD patients. The secretion of some cytokines (IL - 1, IL - 6, TNF et al) in the patients increase which may enhance the respirator}' break - out activity of the inflammatory cells such as neutrophils and mono - macrophages and so on, ROS and RNS produced largely. ROS and RNS may attack any structures and substances in cells including DNA. Among the productions of oxidative injury, 8 - OHdG, for its large quantity of production and less influence factors, is generally considered as the biomarker of the oxidative injury degree of DNA inside of the body. As far as ESRD concerned, at present there are less studies on the oxidative injuries of DNA. This study measures the 8 - OHdG content of serum in order to acquaint the DNA oxidative injury degree in the ESRD patients. Meanwhile it studies whether there are differences in DNA oxidative injury degrees in ESRD which was caused by different etiologies ( chronic glomerulone-phritis and diabetes, mellitus) , and the influences of hemodialysis and antioxidative therapy to DNA oxidative injury. This study also measures the contents of NO and MDA in ESRD patients, and compares with 8 - OHdG to know the correlation between DNA oxidative injury degree and other substance oxidative injury, so as to reach the aim of discussing the mechanism of oxidative injury in ESRD patients andproviding theory evidences for the antioxidative therapy to ESRD.Materials and methords1. Main ragents and apparatus8 - OHdG ELISA kits were provided by Japan Institute For The Control of Aging. The cadmium grains provided by Shanghai Chemical Agents Station. 722 grating spectrophotometer provided by the Third Analysis Apparatus Company of Shanghai. Enzyme - labelled meter (Multiskan Ascent mark) made in Finland.2, Experimental Methods 2. 1 Study ObjectsThere are 4 groups, with 10 cases in each group. Except the normal control group, all the other three are experimental groups. The first group is the normal control group. The second group is ESRD induced by chronic glomerulonephritis without hemodialysis therapy (GN non - dialytic group). The third group is ESRD caused by diabetes mellitus without hemodialysis therapy ( DN non - dialytic group). The fourth group is ESRD induced by chronic glomerulonephritis, which receives hemodialysis therapy ( GN non - dialytic group).Except the fourth group, all patients in the other groups are asked for no smoking histories, no tumor histories, no infection histories in recent one month, no histories of oral intake of Vc, VE, and i-ron agents. The patients in the fourth group all receive the hemodialysis therapies for more than three months. Except histories of oral taking of Vc, VE, and iron agents, the requirements are the same as the other three groups.2. 2 Test MethodsELISA agent kit method is used to test 8 - OHdG. After adding the first antibody fluid, the second antibody fluid sequentially, add chromophore and reaction cessation fluid. Use enzyme - labelled meter to do the quantitative analysis at the wavelength of 450 rim. Modified cadmiuming particle reductive method is used to test NO. Thio-barbital product chromatometry is used to test MDA.3. Statistical AnalysisExperimental data are dealt with SPSS 1. 0 statistical analysis software in order to describe and analyze the t test, and do the correlated analysis of data. P <0.05 means it has statistical meaning.Consequence1. The measure values of 8 - OHdG, MDA, NO in every group.1. 1 The serum level of 8 - OHdG in every groupIn the GN non - dialytic group, DM non - dialytic group and GN dialytic group, the serum levels of 8 - OHdG are all significantly higher than that in the normal control group, P <0.01. But there is no obvious difference between every experiment, P>0.05.1. 2 The serum level of MDA in every group.In the GN non - dialytic group, DM non - dialytic group, the serum levels of MDA are all significantly higher than that in the normal control group...
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