| IntroductionGlomerulonephritis is still the greatest incidence of nephropathy at present in our country. With the progression of diseases, it must be the pathological process in renal scarring (glomerulosclerosis and tubu-lointerstitial fibrosis) , and leads to the end - stage renal dysfiiction. Many cellular and humoral factors are involved in the development and progression of renal scarring. Transforming growth factor - beta(TGF- β is considered to be the main fibrogenic growth factor. TGF -β stimulates the development and progression of renal scarring by many kinds of ways. On the other hand, Myofibroblasts ( Myo - FB ) are thought to be derived from fibroblasts which are stimulated in the in-terstitium by inflammation, immune response and so on, and formed by changes of phenotype. The Myo - FB participated in the fibrotic process in kidney by production of extracellular matrix ( ECM). Myo- FB express alpha - smooth muscle actin (α - SMA) and share the features of smooth muscle cells, α- SMA has been thought as the markers of Myo - FB in tubulointerstitial. To explore the function of TGF -β and Myo - FB in the progress of renal scarring and the expression of them in glomeruli and tubulointerstitial together with their correlation. Renal expression of TGF -β1, α - SMA, and collagen Ⅲ ( COL - Ⅲ) were detected by immunohistochemistry assays in 42 patients with primary glomerulonephritis. Multiple regression analysis wasadopted to study the correlation between the parameters and serum creatinine(Scr) or creatinine clearance ( Ccr) of patients. To investigate the possible links of TGF - β 1 and Myo - FB. It may contribute to the evidence in the renal scarring. Material and MethodsMain ReagentsThe reagent kits of TGF -β 1, α- SMA, COL - Ⅲ and DAB were bought from Wuhan Boster Biological Technology Co, Ltd. Optical microscope was BX60 type Olympus from Japan. Image dialysis system were metamorph image system version 4. 5 from USA.Methods1. SubjectsTotally Fourty - two patients with primary glomerulonephritis who were diagnosised by renal biopsies. Fourty -two patients (22 males, 20 females ) aged from 15 to 60 ( meansS 1.64 11.18). They were divided into four groups according to types of their biopsies. 11 with me-sangioproliferative glomerulonephritis ( MsPGN) ; 4 with membranous nephropathy (MN) ;18 with focal glomerulonephritis (FGS) ;10 with sclerosing glomerulonephritis ( SGN) and the normal control ( NC) is 4 healthy renal tissue.All of patients are primary glomerular disease and excluded the secondary nephropathy resulting from hepatic, pulmonary, cardiac, pancreatic, other infections and systemic diseases. The diagnose standard is concerned with the guide of pathological diagnosis in renal biopsies in the whole country seminar of the 2000 year.2. Immunohistological examinationIrnmunohistochemical staining was performed by peroxidase - labeled streptavidin - biotin technique to detect TGF - β1, α- SMA, COL -Ⅲ on renal tissue slides.3. Semi - quantitatively analysis of immunohistochemical staining According to the intensity and distribution of staining of TGF -βPJ , α - SMA, COL -Ⅲ, the grey were evaluated in the metamorph image system and calculated.4. Statistical analysisDifferences among groups in staining of TGF - β1, α - SMA, COL - Ⅲ and Scr, Ccr were analyzed by the Students t - test. The correlative analysis are performed with SPSS 9. 0 statistics software. The difference is significant if P <0.05.Results1. The expression intensity of TGF -β 1, α- SMA, and COL - Ⅲ in glomeruli and tubulointerstitial1. 1 The expression intensity of TGF - β1In the NC group, TGF -β 1 was not found in mesaginal cells and occasionally in the cytoplasmic area of tubular epithelial cells. There were no significant difference between NC group and MsPGN group together with MN group. With the development of renal scarring, there showed significant difference between NC group and FGS group together with SGN group...
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