| The ischemic cerebrovascular disease ( ICVD) has been commonly seen in the disorders of nervous system in our country and in the world. The incidence and the mortality of the disease have been keeping high. ICVD usually leaves the serious with various dysfunctions. It is the main cause of disability and recognizing. Smoking as a risk factor for ICVD. In recent years, Adhesion molecules and cytokines have played an important role in ICVD. Smoking may damage vascular epithelium cell and cause atherosclerosis ( As). The pathophysiological processes have connection with adhesion molecules and cytokines.Adhesion molecules are caused by cell. Intercellular adhesion molecule - 1 ( ICAM - 1 ) , vascular cell adhesion molecule - 1 ( VCAM - 1). E - selectin, P - selectin have been studied commonly in As and ICVD. E - selectin may mediate the first adhesion of polymorphic neutrophic and endothelia cell. Adhesion molecules have played an important role in ICVD. They promote leukocytes and platelets gathering the endothelial and cause the damage of cerebrovascular. TNF - a is the important factor in the development of adhesion molecules, mediate the adhesion of leukocytes and endothelial cell.In this study, we observed the irnmunoactivities expression of E-selectin and TNF - a in rat brain. Using the rat smoking modelsand immunohistochemical staining. We try to explore the possible mechanisms of E-selectin and TNF - a in ICVD.Matherial and MethodMatherial - Healthy Wistar rats were supplied by Department of Experiment Animals of China Medical University. Cigarettes were obtained from market. Single clone antibody of E-selectin and TNF - a SABC test box were purchased from Wuhan Boster Biological Technology CO. Ltd.Method - Healthy Wistar rats were random divided into six groups of ten animals for each one. (A) the normal control group. ( B) the none - smoking control group. ( C ) short - time smoking group. ( D ) long - time and small - dose smoking group. ( E) long - time and big- dose smoking group. ( F) giving - up smoking group. Ten cigarettes for (C) group, (E) group and (F) group each time. Four - cigarettes for ( D) group each time. Smoking twice one day for each group, and last 30 minutes. Four hours for interval. Smoking three months for (D) group and (E) group, and one month for (C) group. Smoking one month and giving - up one month for ( F) group. Rats were transcardially perfused with heparinized saline and buffered formalin, then brains were removed and fixed with buffered formalin. 5- jxm - thick sections were cut. Immunohistochemical staining and HE staining for E-selectin and TNF - a. Immunostaining was evaluated by light microscopy. The numbers of E-selectin positive vascular endothelial cells were counted in nonoverlapping fields under the light microscope at 400 X magnification. One section from each animals, 5 nonover lapping microscopic fields were collected. With the above methods. TNF - a count was also collected. The results wereexpressed as mean ± standard error. Spss 10.0 was performed to analyse the results of E-selectin and TNF - a. P <0.05 was regard as statistically significant.ResultThe immunohistochemistry stain of E-selectinThe positive cells of E-selectin showed brown and located only in the endothelial cell. There wasn' t expression in the endothelial cells for group A and group B. There was a significant increment expression in the endothelial cells following smoking, the expression in group E was much stronger than those in the rest groups, group C and group D came in second, and that in the giving - up one located in third position.The immunohistochemistry stain of TNF - aThe positive cells of TNF - a showed brown and located neuro-cyte^neurogliocyte and vascular endothelial cell. There are a few expression for group A and group B. The expression in group C, D, E were stronger than group A, B. The expression in group F was lower than group C, group D and group E.DiscussionStudy has already demonstrated that th...
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