Homocysteine Metabolism Abnormality And Pathogenesis Of Pregnancy Induced Hypertension

Objective: To study the relationship among plasma Homocysteine (Hey), folate .vitamin Bi2,C677T mutation of the methylenetetrahydrofolate reductase (MTHFR) gene and pathogenesis of pregnancy induced hypertension(PIH).Methods: Plasma Hey was measured by high performance liquidchromatography and fluorescence detection.Plasma folate and vitamin 612 were determined by automated chemiluminescence system.The mutation of the 677C-*T transition of MTHFR was invetigated by polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) method.Results: The mean level of plasma Hey in the PIH group was10.60?.97umol/l,in control group was 5.96?.50umol/l.It was higher in PIH group man that in control group (P<0.001) .The levels of Hey in the severe PIH group and intermediate PIH group were significantly higher than those in the control group(p<0.001, p<0.001,respectively).The mean level of plasma folate was lower in the PIH group (8.06?.28nmol/l) than that in the control group (15.32?.86nmoiyi) (P<0.001) .There was no significant difference in the mean level of vitamin Bn between the two groups (P>0.05) .There was a negative correlation between the plama concentration of folate and Hey in each group (r= - 0.560, p<0.001; r=-0.572, p<0.001). There was a negative correlation between the plasma concentration of vitamin Bn and Hey in each group (r= -0.305, p<0.05; r=-0.297, p<0.05). The genotype frequencies of MTHFR were 30.1%for TT,61.6% for TC and 8.2% for CC in the PIH group, respectively, while 25.7% for TT,58.1% for TC and 16.2% for CC in the control group. Therewas no significant difference in the frequencies of genotype and alleles between the two groups (P>0.05,PX).05). There was no difference in Hey levels among the three genotype of MTHFR gene in each group (P>0.05JP>0.05).Conclusions: l.Hyperhomocysteinemia is a risk factor of pathogenesis anddevelopment of PIH. 2. The MTHFR gene T677T variant is not the genetic risk factorforPIH. 3.The lack of folate in pregnancy can induce the high level ofHey in plasma, which may be related to the occurrence ofPIH.