| Objective: To assess the association of vitamin D receptor (VDR) gene polymorphism with bone mineral density (BMD) and the effect of vitamin D receptor gene polymorphism to concentration of serum intact parathyroid hormone (iPTH) in control group or chronic renal failure. Methods: We studied 107 patients. There were 56 patients with chronic renal failure and 51 patients with uremia. In the patients with chronic renal failure, there was 32 male patients with 43.9±7.9 year, 172.3±7.4 cm, 75.9±7.6 Kg; and was 24 female patients with 40.3±7.8 years, 158.4±9.7 cm, 65.6±7.4 Kg. In the patients with uremia, there was 28 male patients with 42.5±7.8 year, 172.0±7.8 cm, 73.6±7.1 Kg; and was 23 female patients with 43.2±8.3 years, 158.9±8.5 cm,体é‡64.7±6.0 Kg. There were 60 patients in the control group and there was 30 male patients with 41.1±9.27 years, 172.0±9.2 cm, 72.8±6.0 Kg; and was 30 female patients with 41.9±9.87 years, 160.3±9.8 cm, 64.2±7.1 Kg. Vitamin D receptor gene polymorphism was determined by the Polymerase chain reaction (PCR)Restriction fragment length polymorphism (RFLP) and Apa I enzyme. Bone mineral density was measured by Dual energy x-way absorptiometry. Concentration of serum intact parathyroid hormone was measured by radioimmunoassay. Results: 1 Age, sex, height and weight and BMI show no significant difference among three groups and three genotypes in each group, P＞0.05. 2 Ca++ in the patients with chronic renal failure or uremia is lower than the control group, P＜0.05. And shows no significant difference among three genotypes in each group, P＞0.05. 3 P+++ in the patients with chronic renal failure or uremia is higher than the control group, P＜0.05. And in the patients with uremia is higher than the chronic renal failure, P＜0.05. In the three groups, there was no difference in three genotypes in each group, P＞0.05. 4 The frequencies for Apa I vitamin D receptor gene polymorphism: There were AA type 46.67% (14/30), Aa type 36.67% (11/30), aa type 16.67% (5/30) in male subjects of the control group and were AA type 50% (15/30), Aa type 36.67% (11/30), aa type 13.33% (4/30) in female subjects of the control group. There were AA type 46.88% (15/32), Aa type 31.25% (10/32), aa type 21.88% (7/32) in male subjects with chronic renal failure and were AA type 45.83% (11/24), Aa type 33.33% (8/24), aa type 20.83% (5/24) in female subjects with chronic renal failure. There were AA type 46.43% (13/28), Aa type 35.71% (10/28), aa type 17.86% (5/28) in male subjects with uremia and were AA type 52.1%(12/23), Aa type 34.78% (8/23), aa type 13.04% (3/23) in female subjects with uremia. In the control group or chronic renal failure or uremia, the frequencies for Apa I vitamin D receptor gene polymorphism show no significant difference, P＞0.05. 5 Values of bone mineral density in male subjects of the control group:The lumbar spine were 0.9892±0.065 g/cm2, and there were 1.0786±0.034 g/cm2 (AA type) 1.158±0.076 g/cm2 (Aa type) 1.0863±0.045 g/cm2 (aa type) in three genotypes. The femoral neck was 1.0761±0.034 g/cm2, and there were 0.9763±0.055 g/cm2 (AA type) 1.035±0.052 g/cm2 (Aa type) 0.9798±0.048 g/cm2 (aa type) in three genotypes. The wards triangle was 0.9793±0.046 g/cm2, and there were 0.8653±0.045 g/cm2 (AA type) 1.0090±0.042 g/cm2 (Aa type) 0.8389±0.076 g/cm2 (aa type) in three genotypes; Values of bone mineral density in female subjects of the control group:The lumbar spine was 1.0931±0.087 g/cm2, and there were 1.0895±0.067 g/cm2 (AA type) 1.171±0.053 g/cm2 (Aa type) 1.0981±0.038 g/cm2 (aa type) in three genotypes. The femoral neck was 0.8601±0.059 g/cm2, and there were 0.8543±0.053 g/cm2 (AA type) 1.083±0.062 g/cm2 (Aa type) 0.8790±0.065 g/cm2 (aa type) in three genotypes. The wards triangle was 0.8921±0.043 g/cm2, and there were 0.7642±0.1010 g/cm2 (AA type) 1.002±0.089g/cm2 (Aa type) 0.7692±0.098 g/cm2 (aa type) in three genotypes; Values of bone mineral density in male subjects with chronic renal failure:Thelumbar...
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