| Intracerebral hemorrhage is one of the frequently-occurring disease of the nervous system, which is acute and severe, its rate of multilation and mortality is very high, all these related to the secondary brain edema of perihematoma. Hemoglobin is a kind of neuroeoxicity medium which was produced by lysed erythrocytes after cerebral hemorrhage and contributed to brain edema by leading to a series of pathological changes, such as injurying the nerve cells, damaging the integrity of blood brain barrier, inducing pro-oxidative activity and apoptosis mechanism in the perihematoma and so on. The deleterious effects of lysed erythrocytes (hemoglobin) may derive from the hemoglobin itself, it's breakdown products and upregulated Ho-1 protein levels by hemoglobin, hemoglobin is degraded by Ho-1 in the brain into iron, carbon monoxide and bilirubin. They all contuibute to brain injury and brain edema formation. By creating rat cerebral hemorrhage model, the changes of the brain water and Evans blue contents after rat intracerebral infusion hemoglobin to estimate the damage degree of blood brain barrier and brain edema; by using immunohistochemical technique, the expression of Ho-1 positive cells to estimate the degree of upregulated Ho-1 protein in perihematona; by using conventional pathologic technique and Tunel method to estimate apoptosis cells and brain injury. These help to clarify the mechanism of lysed erythrocytes (hemoglobin) inducing edema after incere-bral hemorrhage. Materials and methods:(1)48 healthy wistar rats were distributed to 3 groups randomly (16 rats each group). Group A: infused saline; Group B: rat self-body-blood ; Group C: antologous lysed erythrocyte (hemoglobin); self-body-blood ; Needles were inserted into the right caudate nuclears using stereo tactic guidance, fifty microliters of saline, autologous lysed erythrocytes N self-body-nonheparinized-fuesh bloodwere infused for Group A> Ek C respectively, 50 microliters of autologous erythrocytes of Group B is equivalent to 30 microliters of autologous hemoglobin. Each groups were infused in 10-15 minutes and detained for 20 minutes until needle was removed.The rats used to observe the permeability of blood brain barrier were given Evans blue solutin (concentration 2.5%, dosage 2ml/kg) from tongue vein after infusion. The rat which were measured the contant of Evans blue and brain water were sacrificed by decapitation at the 24h after the operation. Removed the operation side cerebral hemisphere, cut two coronal slices of brain tissue centered with the needle passage, each slice approximately 1.5mm thick. The slice in front of the needle passage was weighted in the raw condition, immersed in formamide solution (3ml) and was kept at 45 癈 for 3 days and test the solution with violet spectra-meter. The other slices after weighted in raw condition were dry up at 110℃. The difference between these two weight was divided by the raw weight and then the content of brain water was bring out.The rat used to immunohistochemical observation were sacrificed by decapitation at the second day after the operation, the brain tissue was removed, embed in paraffin and made immunhistochemical sections (3 urn thick) using HE staining and immunhistochemical SABC method. The mumber of HO-1 positive cells and the grade of HO-1 positive cells mumber were detected. The data handled with spss 10.0 statistic softmare. The difference of two groups was compared with one-way analysis of variance and rank sum test significant level is a =0.05.Result :(l)After the operation, all the animals behaved low spirit ,low ability of self-clearance and equilibration, eat little, the myodynamia of left extremities decrease and occured spin, etc. some of them sezusing. Groups A also had above-mentioned symptoms, but they recovered at third day after the operation. The symptom of group B and C were worst and developing. Some rats of group C was died. They couldn't recovers until the three day. (2)The results of brain water content: group A 77.15 ± 1.55, B 81.24± 1.32, C...
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