T Helper1,2 Cell Phenotype Markers And Its Cytokines Expression In Peripheral Blood Of The Patients With IM

Objective IM is a kind of limiting disease that is caused by EB virus. Saw more in the child and teenagers,IM have the tendency to break out in recent years, already there was a statement of the causing death IM abroad. Studing its cause , pathology, diagnosing of IM becomes the important subject in the medical circle. Thl, Th2 cell's type offer the new thought in studying immune pathology of infectious disease in recent years. For a long time, the document reports the research in this respect only on the basis of determining their content of cytokines represented. Because cytokines are to exist in the form of network, and a great deal of factors influence its secreted. There is certain limitation in Th1/Th2 function only from analysing its cytokines. Findly , CCR5, CD30 express for Thl, Th2 cell respectively and they are closely related with activating Thl, Th2 . So CCR5, CD30 can be regarded as its corresponding markers of Thl, Th2 .The discoveries of CCR5, CD30 offer direct means for studing Thl/Th2 drift in the body of IM.Method This experiment adopts FCM to determine CD4, CD8, CCR5, CD30 of 40 acute IM,and measure IL-4, IFN- r of liquid in peripheral blood of IM after culturing by ELISA. Do the changes of 30 normal children at the same time and compare the difference of the two.Result1, CD4 positive proportion of IM is lower than the group of NC, P <0.05.1 CD8 positive proportion of IM increase obviously than the group of NC,P <0.01.2, There have difference of IM and NC at CD4/CD8 ratio,P< 0.05.3, CD4, CCR5 express increasely in the group of IM, p <0.01 .4, CD30 + cell express hardly on IM and NC, p> 0.05 .There is no remarkable difference.5 There have difference of IFN- r among IM group and NC group,P< 0.01. 6, There have not difference of IL-4 among IM group and NC group, P>0.05.Conclusion CD4CCR5 positive cell of IM rises obviously. The increase of CCR5 helps Th1 cell to hasten and go back to the nest of Th1 inflammation district and gives to defending the function of the organism. This relate to the fact that EBV infects B, T lymphocyte . CD30+ cell express hardly on IM and NC CD30 .there is no difference in IM group and NC group. It is mainly that Th1 replies the mode of EBV infects early. A large amount of IFN- r are secreted in Thl mode. The result of experiment show that IFN- r are obvious differences in IM group and NC group . IFN- r of patient's group is higher than the normal group.