A Morphological Study On The Effect Of The Treatment Of The Damage To The Internal Organs Of Morphine Dependent Mice By Melatonin

Objective: To observe the effect of the treatment of the damage to the internal organs of morphine dependent mice by melatonin with mcroscope and enzyme histochemistry technology and discuss its mechanism. Methods: 40 male little mice were divided into the experimental group (30 cases) and normal contrast group (Group A, 10 cases) randomly, Experimental group was also divided into three groups, morphine dependent group (Group B), morphine withdrawal group (Group C), MT group (Group D), each of which had 10 cases. Group B, C, D was given subcutaneous injection of 70mg.kg-1 (8:00, 16:00, S.C.) morphine for 4 weeks, Group A was given subcutaneous injection of equivalent physiological saline. Group B continued to be injected morphine after 28d, Group C give up naturally, and so did group D, of which their stomach were irrigated with melatonin of 50mg.kg-1 (once a day), and Group A and Group Cwas irrigated of equivalent physiological saline until 56th day the experiment was finished. The liver, kidney, prostate, and testis of the animals were extracted and made into slices with paraffin wax buried, and HE-dyed, and icy slices with enzyme histochemistry dye. Observed with microscope and analyses with computer imaging analyses system. Results: The tissues of liver, kidney, prostate and testis are all normal with group A. The incidence of changes in liver tissues of Group B was 100% (10cases) with a rise in ACP activation and a drop in SDH activation; And that of Group C was 90% (9cases) with a rise in ACP activation and a drop in SDH activation; And that of Group D was 10% (only 1 case) with the normal activation of ACP, SDH. The kidney tissue of group B had some cell changes with a rise in ACP activation and a drop in SDH activation; The changes of the kidney tissue of group C and D had not observed obviously, but Group C had a rise in ACP activation and a drop in SDH activation; And that of Group D had normal activation of ACP and SDH. The prostate and testis tissues of Group B withered with an increase in ACP activation and a decrease in SDH activation, and that of Group C had slighthyperplasia, the prostate had an increase in ACP activation and a decrease in SDH activation, the testis had a decrease both in ACP and SDH activation, while that of Group D were normal basically with the normal ACP and SDH activation. Conclusion: 1. Morphine dependent can lead to the structures of the prostate, testis, liver and kidney of mice be damaged and their cell SDH activation was increased and ACP activation was decreased. 2. Melatonin (MT) can make the damages to the structures of the prostate, testis, liver and kidney of morphine dependent mice restored to normal and helps their cell SDH and ACP activation return to normal.