| OBJECTIVE: Probing into the mechanism of Phenytoin Sodium protective effect on brain tissue in cerebral ischemia reperfusion. METHODS: Rats were subjected to cerebral ischemia by bilateral ligation of the common carotid arteries for 45 minutes and then reperfusion for 2 hours. Forty rats were randomly divided into four groups:sham group (group A) undergoing all surgical procedures but ischemia;normal saline group(group B);phenytoin(15mg/kg) group (group C); phenytoin(30mg/kg) group (group D).After completing the required procedures, the samples of brain tissues were taken to measure the activities of ATPase and mitochondrial succinic dehydrogenase (SDH), to observe the expression of Fas protein and the ultrastructure of cortex, furthermore phenytoin's influence on above indexes was observed.RESULTS: We can see from all indexes, the activities of ATPase and SDH was significantly inhanced in group C and D compared with group B (p<0.05), and there was no statistic difference between group C and D; and the the expression of Fas protein was significantly reduced in group C and D compared with group B(p<0.01). And the ultrastructure of cortex was improved in group C and D.CONCLUSION: (1) Using Phenytoin Sodium have protective effect on brain tissue in cerebral ischemia reperfusion, and there is no statistic difference between two different doses of Phenytoin Sodium. (2) the mechanism of Phenytoin Sodium protective effect on brain tissue in cerebral ischemia reperfusion is to block the Na+ channel, inhance the activities of ATPase and to decrease the EAA excititoxity and protect the function of mitochondrion etc.
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