Study On Cytocompatibility And Genotoxicity Of Regenerated Silk Fibroin Films

Biomaterials used for repair of parenchyma, such as skin ,tendon, esophagus, repratory, breast etc, probably have privilege in repairing and reconstruction compared to inertia materials,which be compatible with tissue , degradated and absorbed in vivo. Biomaterials should have histocompatibility and hemocompatibility, phisical and chemical properties accordingly as so. The materials /cells interaction is one of the important researches on biomaterials.lt possesses bright future in the application of biomaterials as the regenerated silk fibroin films(RSFFs) share some excellent characters such as non-toxicity, non-stensitization and osmosis. The study on cytocompatibility and genotxicity of RSFFs plays a significant role in its modification and application.Part I Effect of regenerated silk fibroin film on culture of rat embyro dermal fibroblastsAbstract: Objetive: To investigate the effect of regenerated silk fibroin films (WL,SD,HY) on the attachment and growth of rat embyro dermal fibroblasts. Methods: Rat embryo dermal fibroblasts were cultivated on films and extractions of films in vitro. To observe cell morphology on films ,the growth in porous silk fibroin sheets with Scanning electron microscope (SEM), activity of cell prolifevation and relative growth rate(RGR) with a MTT assay. Results: The influence of cellular attachment and morphology was not evident .No significant diference was observed compared to control,as well as the normal growth curves .However ,the cell growth was suspressed in HY group.Conclusion: WL ,SD regenerated silk fibroin film is more cytocompatible with rat fibroblasts,while HY is toxic to cells and should be modified to be compatible with cells.