Distribution Of Adriamycin Contained By Galactosed Albumin Magnetic Nanoparticle In Rats

Object: to investigate distribution of adriamycin contained by galactosed albumin magnetic nanoparticle injected by lingual vein in rats.Methods: All animals were divided into four groups which were A group: adriamycin; B group: adriamycin contained by magnetic albumin nanoparticle ; C group: adriamycin contained by galactosed albumin magnetic nanoparticle; D goup: adriamycin contained by galactosed albumin magnetic nanoparticle plus external magnetic field. Each group included 15animals, which were subdivided into 5 subgroups, thus each subgroup included 3 animals. The drugs were administrated into corresponding rats at a dosage of adriamycin 2.5mg/kg body weight. Before drugs were injected intravenously, animals were explored abdominal wall along middle line of abdomen , the left hepatic lobes of animals in D group were exposed ,which magnetite were placed on. Animal were sacrificed 5min 15 min 30min 60min 120min after drugs were injected. Blood heart lung liver kidney were harvested, targeted Liver or untargeted liver in D group was harvested. Blood samples were made into plasm, organic tissues were rinsed for homogenate. Adriamycin was pick up by hydrochlore-ethanol method, which level was measured by fluorescence photometer.Results: Adriamycin level of targeted liver tissue in D group was the highest in all groups, and not only singnificantly higher than that of untargeted in D group , but also that in A B C group(p<0.05); Butadriamycin level was not significantly different between targeted liver tissue in D group and liver tissue in C group(p>0.05) Adriamycin level of plasm heart lung kidney was not significantly different between C and D group(p>0.05), but significantly lower than that in A and B group(p<0.05) Adriamycin level of spleen in B group was the highest in all groups, but that was not significantly different in A C and D group(p>0.05) drug targeting index and drug selective index were increasing in C and D group as tested time went.Conclusions: After adriamycin contained by galactosed albumin magnetic nanoparticle was administrated intravenously, adriamycin can be targetedly deliver to liver, and adriamycin level in plasm heart lung kidney spleen was significantly lowered in extra-hepatic organ, external magnetic field can significantly increase adriamycin level in specific site of liver tissue. So it was feasible that adriamycin contained by galactosed magnetic albumin nanoparticle was administrated intravenously.