The Inhibitory Effects Of Non-Steroid Anti-Infammatory Drugs In SKOV3 Human Epithelium Ovarian Cancer Cell Line

Objective:The purpose of this study was to investigate the cellular effecs of Acetylsalicylic acid (ASA),Acetaminophen, and a selective COX-2 inhibitor(Nimesulide) on the growth of SKOV3 cell lines of human ovarian cancer in vitro.Method:SKOV3 cell line was cultured with ASA, acetaminophen and Nimesulide. The cell proliferation was detected by MTT assay; Cell cycle repartition was analysed by Flow cytometry (FCM); The morphologic change and apoptosis of the cell were assessed under the Electron Microscopy(EM). Immunohistochemical assessment was performed for alterations in expression of COX-2, Ki-67 and C-erbB-2 enzyme. Results:A decrease in cell number compared with controls was observed for all of the cell line treated with ASA, Acetaminophen and Nimesulide by MTT; the inhibition of cell proliferation was time-dependent and dose-dependent fashion.COX-2 was presented positive in SKOV3 cell line and the expression of C-erbB-2 were decreased following treatment with ASA; and the reduction of Ki-67 were shown in Immunohistochemical assay in the Nimesulide group.The cell apoptosis and necrosis were detected with ASA,Acetaminophen and Nimesulide through EM. The cycle of cell was retention in G0/G1 by Nimesulide (400μM/L) and ASA (10－2M/L)treated.Conclusion:(1)The cell proliferation was inhibited by ASA,Nimesulide and Acetaminophen. (2)ASA suppresses SKOV3 cell growth and induces apoptosis by inhibiting C-erbB-2 expression.(3) Nimesulide suppresses tumor growth and induces apoptosis by inhibiting Ki-67 expression.(4)The most possible anti-tumor mechanism of Acetaminophen was to restraint the action of FSH. Summary: Our suggest additional study for the use of NSAIDs, specifically COX-2 inhibitors, as a stategy of chemoprevention for ovarian cancer.