Experimental Studies On Derived Material Of Natural Bone And Mouse Marrow Stromal Cells

Objective: we prepared a kind of derived material of natural bone , and we cultured the mouse marrow stromal cells with this kind of material, observed the combined culture process with the microscope, to understand the consistent and the adhibit character. We want to find a kind of plank material that has bright clinical future and can be fit for the tissue-engineered bone constuction.Method: we made the bone pieces from the New Zealand rabbits' limb bone. The bone pieces were treated with H2O2 and aether, then sterilized and stored by 80℃ under zero. The BMSCS were isolated and purified by gradient centrifugation in Percoll. The BMSCS were collected and cultured in DMEM(LG) with 15% fatal bovine serum(FBS).Cultures were maintained at 37℃ in humidified atmosphere chamber containing 5% CO2 . The adhibit cells kept for passage. The five passage cells were digested by trypsin and the number of the cells were adjusted to about 1×106 /ml. The cells were dripped and filtered into the derived material's coarse faces. Then the cells and the derived material were cultured together with 15% fatal bovine serum(FBS). We observe the combined culture process with the microscope, to understand the consistent and the adhibit character. results: 1,we observed by SEM that the biological bone material has the original bone tissue's reticulation hole structure system and the original bone tissue plank's three dimension holes system. 2, The BMSCs cultured were grown like fibroblast, the figure of the BMSCs were in focus, the cell plasm refract well, gradually gather together ,this can explain that the BMSCs grew bloom. 3, The third day we saw the cells crept from the biological bone material, after several days the cells on the derived material increased, and grew together. We observed by SEM that the biological bone material has the coarse surface and crannies and microholes. The BMSCs adhibited the bone material or grew to the crannies and the microholes. The BMSCs had the shuttle figure or polygon with the threadlike prominency. Conclusion: The derived material of natural bone can be got easily and has many sources. It has the three dimension hole stucture and dynam intension, and has good biological consistent character and the material-cell border; The BMSCs can split and proliferate on the plank, this proves that the material has a bright clinical future can be fit for the tissue-engineering bone.