Epidemic Feature Of Anthrax In Liaoning Province And Detectation Of Bacillus Anthracis From The Polluted Soil

Objective1. We analyze the anthrax cases of liaoning history and investigate the outbreak of anthrax in 2001, which reveals the epidemic feature of anthrax and its epidemic factors , the result will provide theoretically basis for taking the measures of prevention and control of anthrax ;2. Investigating the polluted condition of anthrax in soil samples ;3. Detecting the virulence of the Bacillus Anthracis, in the same time the effect of sterilization is also evaluated.Methods1. Epidemic investigational method ;2. Traditional seperated and conforming method;3. Plasmid extraction and PCR amplication.Results1. The epidemic period of anthrax in liaoning province is 8 -10 years ,the farmers are the mian part of the totol cases , which occupy the 80. 20% ;and simple skin anthrax cases are the majority , whi,ch hold the 90. 62%. The anthrax cases of intestines are easy to die . The epidemic factors show that the most cases have had contaced with the silk or dead anamals. 2. 5 strains have been i-dentifled from 34 soil samples , which we obtained in three times , though the latter soil samples have been sterilized . PCR amplication indicates that the 5 strains all have capsular and lethal factor genes.Conclusion1. The epidemic period of anthrax in liaoning province is 8 - 10 years ,the farmers are the hignly dangerous population , and simple skin anthrax cases con-stitude the majority. The anthrax cases of intestines are easy to die . The epidemic factors show that the most cases have contaced with the silk or dead ana-mals ;2. The soil has been polluted by Bacillus Anthracis , the animals are likely to take the Bacillus Anthracis in their mouth when they eat the grass, and their dead bodies can pollute the environment again , that is to say , the potential harm is very serious; 3. Bacillus Anthracis has been seperated from the sterilized soil samples and PCR amplication indicates that the strains are all virulent isolates , which shows that the effect of sterization is not reliable;4. PCR can avoid the second polluted questions of using animals to detect the virulence of Bacillus Anthracis.