The Elementary Study Of Tooth Regeneration With Dental Pulp Stem Cells

The long-term goal of tooth regeneration is to create human teeth, and eventually, to enable patients to regenerate their own teeth following tooth loss using their own tissues. It was a long-term project, so we only did the early study and elementary exploration about tooth regeneration with dental pulp stem cells (DPSCs). In this study, we established a feasible technique for tooth regeneration in mice by using culture cells. We demonstrated that human tooth morphogenesis was similar to mice. It means that the molecular mechanisms of murine tooth development might be applied in human. Human tooth germ grew well beneath kidney capsule of athymic mice, which indicated that athymic mice were suitable host for human tooth germ development. DPSCs isolated from adult dental pulp exhibited a high proliferation rate, and have the ability to form calcified nodules, In tissue-recombination experience, DPSCs were recombined with the human dental epithelium, and the human dental epithelium was cultured with the mouse dental mesenchyme. However, the recombinants both failed to form tooth in the experience. They usually formed the structure of bone-like tissue. But the results needed to be further confirmed.