The Study On The Effects Of Nanomaterial To Cells In Vitro

Objective To study the security of silver nanomaterial and rare earth nanomaterial to cells in vitro in order to provide the theoretic and practical foundation for the application of nanomaterial.Method The dose-dependent manner were investigated by MTT assay, and the time-dependent manner were tested by cell-counting method. The nanomaterials and cell components were observed by a transmission electron microscope(TEM).Result (1)There was no significant difference among the survival rate of each concentration for silver nanomaterial (P>0.05) . There was significant difference among the survival rate of final concentration range ( 0.016 -0.5mg/ml) for rare-earth nanomaterial in a dose-dependent manner(P<0.05). (2) In various time for silver nanomaterial ,there was no significant difference between the number of living cells in experiment group and that in control group (P>0.05 ) .But for rare-earth nanomaterial, the number of living cells in experiment group was significantly lower than that in control group(P<0.01).(3)The distribution of nanoparticles in the cells was observed using a TEM. The silver nanoparticals existed not only out of cells, but also in the cytoplasm. Some silver nanoparticles dented the nucleus. But karyotheca and other components weren't damaged .Rare-earth nanoparticals didn't exist in the cytoplasm, but some necrocells were seen.Conclusion (1)The silver nanoparticals exist not only out of cells, but also in the cytoplasm. Some silver nanoparticles dent the nucleus. It need research further to if silver nanoparticles may exist in the cell for a long time or cause mutation and other diseases. Rare-earth nanoparticals doesn't exist in the cytoplasm, but cause necrocytosis. (2) Silver nanomaterial beyond the antibacterial concentration of silver nanomaterial doesn't affect the activity of WI -38 cell and Hela cell , but rare-earth nanomaterial under the minimum antibacterial concentration of rare-earth nanomaterial can inhibit the activity of WI -38 cell and Hela cell strongly. It shows that the silver nanomateril has a good biocompatibility ,and would provide an intensive theoretic foundation for the application of silver nanomaterial to biomedicine. But rare-earth nanomaterial doesn't.