Functon And Relation Between Apoptosis And Parp Or Calpain After Cerebral Ischemia

Aim The study is to explore the expression of poly (ADP-ribose) polymerase (PARP) and Calpain in focal cerebral ischemia with reperfusion injury and observe morphological features of apoptotic cells and the role of PARP and Calpain in apoptosis.Methods 36 adult female Wistar rats were subjected to middle cerebral artery occlusion (MCAO) and different time of reperfiision. Rats were randomly divided into MCAO groups and sham group that were same as operation, but no MCAO. To explore the implication and the kinetics of such event in apoptosis induced insult induced by MCAO in situ TUNEL technic. To observe the expression of PARP and Calpain at the different time points and then assess their function in apoptosis induced by MCAO in situ hybridization technicResults Apoptotic cells lie in the boundary zone to the ischemic core. The number of apoptotic cells in the ischemic core was less than ischemic penumbra (p<0.05) except for 2 h, 7 d, 14 d. Situ hybridization demonstrated the evident expression of PARP at 2 h. The peak of PARP was first detected at 2 h in the corpus striatum and then cerebral cortex at 6 h . With increasing length of survival, the expression of PARP began to decrease, then dropped a very low level at 14 d. But there are difference between MCAO group and sham control (p<0.05). Situ hybridization demonstrated the evident expression of Calpain in the cerebral cortex and corpus striatum at 2 h. The study was detected a bimodal pattern of Calpain with an initial increase at 2 h followed by a more prominent secondary increase at 12 h after reperfusion. After 24 h Calpain began to decrease, then dropped a very low level at 14 d. But there are difference between MCAO group and sham control (p<0.05).Conclusion Apoptosis lie in the boundary zone to the ischemic core. PARP and Calpain play an important role in apoptosis. The inhibition of PARP and Calpain can prevent and cure cerebral ischemic damage.