The Pharmacological Study Of YKD Granule

Objective:To observe and assess the pharmacological effect of Yunkeding Granule(YKD) on Meniere's disease (MD). Methods:1 .Using the damage model of membranous labyrinth sensor induced by chloroform to observe the effect of YKD Granule on nystagmus as well as head swing and circumrotating.2.Using the model of Endolymphatic hydrops induced by surgery, to study the therapeutical function of YKD Granule on the morphology of inner ear. SMA increasing rate was also examined.3.The effect of YKD Granules on the pigeon vomiting induced by bluestone as well as by cisplation was also studied.4.The effect of YKD Granules on the mouse dormancy induced by low dose sodium pentobarbital was observed too.5.To study the influence of YKD Granules on the abdominal capillary permeability, Evan's blue injection was adopted.6.Using rat metabolizing baskets, the effect of YKD Granules on the urine output was observed.7.The effect of YKD Granules on mouse spontaneous activities was analysised. 8.To look into the effect of YKD Granules on the cloning of BMDC in vitro and its MHC Class II molecule expressin, we adopted a new method of the serum pharmacology. BMDCs were isolated from C57BL/6J mice and cultured in RPMI1640 complete medium with GM-CSF plus IL-4. BMDCs were grouped:(1)normal control;(2) control serum, additionally adding blank serum; (3) Drugs containing serum,additionally adding blank serum. The different morphology characteristics of BMDC indifferent stages were observed with microscope and Gimesa' dyeing as well asimmunocytochemistry analysis.Results:1 .Chloroform damaged the right membranous labyrinth sensor.The Guinea-pigs appeared nystagmus and circumgyration as well as head swing.Compared to control group animals ,YKD Granule (8, 16g/kg) could obviouslyreduce nystagmus degree (P<0.05), but had no effect on circumrgyration and headswing.2.The model of, Endolymphatic hydrops was successfully made by surgery.Compared to control, Guinea-pigs of surgery model group were found that theirvestibular membranes were obviously protruding and stria vascularis were abnormitytoo.However the vestibular membranes of animals i.g.with YKD Granule were slightlyprotruding and the morphological characteristics of stria vascularis was also resumed.Moreover it was founded that the difference of SMA increasing rate between groupswas remarkable (P<0.01).3.Both bluestone and cisplation could induce pigeon vomiting. YKD Granules couldobviously reduce vomiting degree and prolong the delitescence4.YKD Granules ( 5 10 20g/kg)could shorten the time span before dormancy inducedby sodium pentobarbital and prolonged dormancy lasting period at the dose of 1020g/kg i.g. (compared to control: P<0.05 or P<0.01).5.After Evan's blue injection , the absorbency of abdominal washing fluid wasexamined at 590nm. Compared to control we found that YKD Granules (10 20g/kg )could restrain the increase of abdominal capillary permeability (P<0.0l).6.YKD Granules was observed to have no effect on the urine output by using ratmetabolizing basket, The difference in groups was not statistically significant. . (compared tocontrol: P>0.05)7.YKD Granules (5 , 10, 20g/kg ) could significantly cut down mouse spontaneousactivities such as walking and lifting forelimb.8.Both control serum and drugs containing serum could accelerate the differentiationand maturing of BMDC, inducing BMDC with many more long processes and I-Aexpression by immunocytochemistry analysis. But there was no obvious differencebetween control serum and drugs containing serum.Conclusion:YKD Granules had obvious effect of calmness and suppressive function on vomiting.ltcould counterwork some symptoms of model animals with Endolymphatic hydrops byreducing abdominal capillary permeability and lighting the degree of endolymphatichydrops and so on. But it perhaps had no function to the immunological mechanisms in thepathogenesis of Menie...