Effect Of 2β-(3-Hydroxypropoxy)-Calcitriol (ED-71) On Proliferation And Differentiation In Osteoblast-like Cells

Objective: The ability of2 β -(3-hydroxypropoxy)-Calcitriol (ED-71) to affect cell growth and differentiation in human osteoblast-like cell line OS-732. Methods: Osteoblast-like cells were cultured with various concentration of ED-71. The Effects of ED -71 on proliferation and differentiation were detected by MTT,.cell cycle, alkaline phosphatase activity and level of osteocalcin (bone gla protein, BGP). Results: The proliferation rates of osteoblast-like cells cultured for 4 days were significantly inhibited by 10-7 mol/L concentration of ED-71. ED-71 in 10-8 ,10-9mol/L concentration can significantly increase BGP level, butED-71 in 10-7mol /L concentration may decrease BGP level. Alkaline phosphatase activity compared with control is not significant. So, the effects of ED-71 on cell differentiation in osteoblast-like cells is not identical. Conclusion: ED-71 can inhibite cell growth in high concentration and improve cell differentiation in lower concentration.