Expression Of P27～(kip1) Protein In Nephroblastoma And Its Significance

Objective: To investigate the expression of P27 in nephroblastoma, and the relationship between its expression and prognosis of the patients as well as clinic pathological characteristic. Nephroblastoma is one of the most common malignancy solid tumor in children, rare in adult. Nephroblastoma may come from metanephrogenic blastema's undifferentiation. It may be hereditary or non-hereditary. The age of hereditary nephroblastoma is less. All the bilateral nephroblastoma and 15-20% of unilateral unilateral nephroblastoma is hereditary. The forming and prognosis of tumor is closely related to adjustment disorder of cell cycle. Cyclin and CDKs are positive adjustment factor of cell cycle, and CDKi is negative. P27kip1 is thermal stability protein whose molecular weight is 27KD. P27kip1 is discovered by Polyak in 1994. P27kip1 is major inhibitor of CDK2/cyclin E and CDK4/cyclin D. At present, P27kip1 may directly affect adjustment point of Gap/Synthesis, and resist cell proliferation and forming of tumor. Presently, most research about P27kip1 has been done abroad, but seldom in China. Now most scholar think that P27kip1 is related to non-small cell lung cancer,breast carcinomas and coloreactal carcinomas. P27kip1 expresses differently in different tumor tissue. P27kip1 is considered an independent marker of tumor.Methods:The expression of P27kipl in 44 cases of nephroblastoma was detected by immunohistochemical method. Anlysis the relationship between P27kip1 expression and prognosis of the patients as well as clinic pathological characteristic. Discuss the function of P27kip1 in forming and development of nephroblastoma. All patients were followed up. The univariate and multivariate prognostic analyses were performed by using Cox regression model.Results: The high expressive rate of P27kip1 for nephroblastoma was 65.91%(29/44), the low expressive rate was 34.09%(15/44). Low P27 expression in nephroblastoma was significantly associated with late stage , local recurrence , and high incidence of lymph node metastasis, But high P27kip1 expression is contrary. All patients were operated and recepted chemotherapy. Vincristini Sulfas ,Cytoxan and Dactinomycin were used. The course of treatment was 6 months to 1 year. 2 cases received radiotherapy. 3 1 cases were followed up. 3 cases died.Conclusion:. P27kip1 is an independent and valid prognostic marker of nephroblastoma .