The Preliminary Study Of Histamine On T Cells In Vitro

Objective To investigate the influence of histamine-the new type of signal transducer, on T cells and the mechanism. Methods Density gradient centrifugalization and absorption technique were respectively applied to detach peripheral blood mononuclear cells(PBMC) and peripheral blood lymphocytes(PBLC). CD4+and CD8+ T lymphocytes separated with anti-CD8 and anti-CD4 antibody were cultured in vitro. The proliferation rate was detected by MTT methods and IL-2 levels in supernatant were quantified by enzyme linked immunosorbent assay (ELISA). Result: EL-2 levels in supernatant and proliferation rate of CD4+ and CD8+ T lymphocytes treated with histamine were significantly lower than those of their corresponding un-stimulated controls(P<0.05). IL-2 production and proliferation rate of CD4+ andCD8+ T lymphocytes co-cultured with histamine and cimetidine were higher than those of the controls cultured with histamine alone (P<0.05). CD4+ T lymphocytes produced much more IL-2 than CD8+. Conclusions Histamine inhibits T lymphocytes proliferation and IL-2 production, and the inhibitory effect can be stocked bycimetidine. CD8+ T lymphocytes also secret IL-2, but less than CD .