Effect Of Liver Targeting On Adriamycin Polybutylcyanoacrylate Napariticles Diameter Of Nanoparticles

Objective:To observe the difference of the bio-distribution in the body of the rats of the adriamycin polybutylcyanoacrylate napariticles(ADM-PBCA-NP) freeze-dry dose for the different diameter of the napariticles and the solution of the adriamycin (ADM) rejected by the vein , and study the liver targeting for ADM-PBCA-NP with different diameter of nanoparticles to normal liver,and choose the ADM-PBCA-NP for the best liver targeting.Methods: ADM-PBCA-NP with various ranges of diameter of the nanopartices including (22.3?.2)nm,(48.6?.2)nm,(101.9?20.3)nm,(143.5?3.5)nm and (194.2?8.4)nm were produced by using the method of emulsior polymerization based on the carrier materials of a -polybutylcyanoacrylate. 180 rats from kunmin were divided into 6 groups at random(ADM-PBCA- NP with diameters of the nanoparticles were divided into 5 groups, in addition free Adriamycin was considered as comparative group) ,with 30 rats for each group, ADM-PBCA-NP freeze-dry dose and ADM solution were injected into the veins of rats by the formulation of 2mg/kg.5 rats in each group were killed 5 , 15 ,30min , 1, 5 and 12 hours respectively after drug was given , ADM concentrations were determined using a high effective liquid chromatography with fluorescence detector technique, when obtaining the samples from liver, kidney, spleen, heart, lung and plasma respectively.Results: The ADM concentrations in the lever of rat in all the napariticles groups is markedly higher as compared to free ADM groups during 12h, with the excepting of the group with (22.3?6.2)nm(P<0.05), whereas the concentration in the heart muscle wasnot determined or markedly lower than free ADM group(P<0.05), high concentration in kidney did not keep long time; ADM concentration in the lung in the group of (194.2?8.45)nm was higher than that of (48.6?.2)nm (P<0.05). ADM concentration in the group of (101.9?8.3)nm in the liver of the rats were remarkably higher than that in the other groups (P<0.05),the second is (143.5 + 21.4)nm. ADM concentration in the group of (22.3 + 6.2)nm was not determined at all in the liver ,kidney ,heart ,spleen and lung.Conclusions: ADM-PBCA-NP with some different ranges of diameters of the nanoparticles has the better liver targeting ,and the characteristic of low releasing , which decrease the medicine distribution in the heart and other tissues.So it's a good medicine-diverting system for treating liver cancers.DM-PBCA-NP with the diameter of 100-150nm has the best liver targeting ,and this supplies experimental data for producing na-meter medicine-carrier of polybutylcyanoacrylate for treating liver cancers .