A Study On The Effects Of EGCG On Invasion And Metastasis Of Breast Cancer Cells And Its Possible Mechanism

Objective: To study the effects of tea extract EGCG on invasion and metastasis of breast cancer line MDA-MB-231 and the possible mechanisms. Methods: Our study was conducted on a highly metastatic human breast cancer cell line MDA-MB-231. Taking benzyl-α-GalNAc(a glycosilation inhinbitor) and RPMI-1640 group as positive and negative control respectively. MTT is performed to determine the growth inhibiting effect of EGCG for getting its IC50. Expression of MUCI was observed by immunocytochemistry. Adhesion of MDA-MB-231 to Matrigel was measured with MTT assay. Invasion and metastatic potential were evaluated with transwell chamber. Collagenase-Ⅳ secretion was dectected with SDS-PAGE before and after the administration of EGCG . Results: Our study demonstrated that EGCG had a time/dose- dependent growth inhibiting effect on MDA-MB-231 and the IC50 was 72.45μg/ml. Compared with untreated group, MDA-MB-231 treated with 36μg/ml EGCG displayed decreased MUCI expression (64% vs 100%), while MUC1 expression was not significantly different between benzyl-α-GalNAc group and EGCG group. Compared with untreated group,Adhesion is remarkably decreased. Invasive and metastatic abilities of three groups are 85.5±14.9,64.7±1.2,40.9±10.8 and 109.0±8.30,76.2±10.69,50.8±5.30 respectively,the Collagenase-Ⅳ secretion of MDA-MB-231 cells was also decreased.Conclusion: In vitro, EGCG has a time/dose-dependent growth inhibiting effect on MDA-MB-231 cells. EGCG may suppress adhesion , metastasis, Invasion, and Collagenase-Ⅳsecretion of MDA-MB-231 cells by inhibiting the production of MUC1 .