| IntroductionThrombosis is the occlusion of cerebral arteries caused by certain reasons that can lead to ischemia , hypoxia , soften and necrosis of cerebral tissues . It is the most common type of cerebral vascular disease with high mortality and mobility . Thrombolysis is the most effective therapy . However , ischemia -reperfusion damage happened during the course of thrombolysis is difficult to solve because the mechanism is still unclear . Generally speaking , in ischemia insult the exhaustion of ATP may affect the function of mitochondroma , lead to aggregation of intracellular Na+ , Ca+ + and free radicals. Then lots of enzymes are activated , which can break the cell skeleton and membrane and lead to degradation of DNA. Thus the death of neuron is inevitable. The mechaniosm of ischemia - reperfusion damage is a very complicated , mutifactor process , including stress reaction , Inflammatory reaction , overload of Ca+ + , cerebral edema and aptosis . ADM is a newly discovered mutipeptide consisted of 52 amino acids , belonged to calcitonin - gene related peptide family . It is believed that ADM has relationship with ischemia - reperfusion damage in many tissues including brain . We observe the expression of ADM in normal cerebral tissue and variation of ADM after ischemia - reperfusion damage in focal brain ischemia -reperfusion model of mouse by immunochemistry method to evaluate the relation between ADM and brain ischemia - reperfusion damage . ischemia - reperfusion damage.Materials and Methods1. Materials1. 1 Reagents: Immunohistochemistry, using SABC method, first antibody is rabbit anti mouse ADM monoclonal antibody ( working concentration is 1:320). SABC kit.1. 2 Animal and other materials: S - D rats and so on.2. Methods2. 1 Animal grouping ;S -D rats were distributed to three groups at random and that is normal control , sham operation and CIR group.2. 2 Making rat CMAO model by using Zea Longa modified way.2. 3 Detecting the level of ADM expression through immunohischemistry.Result1. The encephalic tissue section HE staining observed in light microscope The encephalic tissue cortical cells have normal form and structure in control , sham operative opposite side of ischemia groups. Neurons decrease, put in disorder, the peripheral diastema of cell widen. A lot of neurons become ischemia and necrosis, cytoplast swelling , cytoplasm staining blandly, karyoplast dissolve and structure not clear in frontal and parietal lobe cortex , infracortical medulla and basal ganglia region,and have many contract neurons in infarct peripheral region of ischemic reperfusion side.2. The expression of ADM after cerebral ischemic reperfusionThere was the expression of ADM in the normal groups. There is no different brtween the normal group and sham - operated group . The expression of ADM reached the peak after ischemia - repurfusion 6 hours , lasting one week .DiscussionADM is a polypeptide consisted of 52 amino acids that can relax vessels . Itis mainly originated from cells of endothelium and smooth muscle of blood vessels . Using immunoradical method , Kitamura K found ADM in plasma , lung , kidney and adrenal gland ,but not in brain .Recently Satoh observed the existence of ADM in every region of human brain with a more sensitive and specific immunoassay method . The content of ADM in thalamencephalon was the highest , and next to thalamencephalon was hypothamalus and pon . While cerebral cortex , cerebellum and medulla equina had relatively low content . The wide expression of ADM mRNA was consistent with the distribution of ADM immune positive cells in the brain of mouse. Brain can not only synthesize ADM , but also secrete and release ADM to regulate the function of cardiovascular system.Weils work showed ADM immune positive neurofibers and neurons distributed widely in the mouse'brain. Immunogenic ADM existed in all the regions of the brain . Our result also showed the distribution of ADM immune positive cell in nomal brain tissue and the regio...
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