| PrefaceThe chuanhuning injection is a pure herb, preparation. Its main component is Potassium Dehydroandrographolide Succinate. This drug has effective antipyretic effects on fever induced by bacteria or endotoxin. Whether this herb exerts its antipyretic effects through changing the level of neural mediators of fever, and whether PGE2 and cAMP play a role in its .antipyretic effects has not yet reports up to present. In our study we used dry yeast to making fever model, through maturating the content of PGE2 and cAMP in hypothalamus of rats and from the point of view of neural mediators of fever we studied its antipyretic mechanism of chemical information transfer. This will introduce experimental thereunder and theoretic base for clinical use and further usage.Materials and Methods1. animals and temperature surveyHealthy male Wistar rats were kept in our experimental environment 3 days before the experiment. The temperature was kept at 20 ?1X.. In our work we put the rats into special cages and the probe of the thermometer was put into the rectum for 6 cm. We took the mean value of two temperature before experiment as the normal temperature (if the differences in temperature were more than 0. 3C then the rat was knock out).2. experiment group and disposal(1) control group: no treatment; ( 2 ) model group: After measuring thenormal temperature the suspension of yeast was injected hypodermically(10ml/ kg ). ( 3 ) herb group 1: 240min after making model the celiac injection of chua-nhuning was administered ( 125mg/kg ). (4) herb group 2: the method is the same as last group , the dosage is 250mg/kg. The rats' temperature of every group was noted every 15min from 180min after recording normal temperature until 360min(the point is peak period of the fever induced by yeast) , then rats were decapitated quickly and hypothalamus tissues were taken out and put into liquid nitrogen for fixation, 20min later the samples were transferred to refrigerator (-70C).3. distillation and assay of the content of PGE2 and cAMPTake some hypothalamus tissues, then we began to assay the content of cAMP and PGE2 according to specification of the Test Kit.4. observation and statistical analysisobservation item: (1) the mean temperature curve (360min). (2) differences in temperature at peak time(360min after yeast injection). (3)the content of PGE2 and cAMP. Statistical analysis; all data were mean + SD. The differences between groups were assessed by student' s test, and the correlation was evaluated by correlation analysis.Results1. the effects of chuanhuning injection on fevered rats caused by yeast The temperature of control group did not change magnificently during theexperiment. The model group animals' temperature raised significantly which was more higher than control group ( P < 0. 01 ) ; and the peak time is at 360min. Compared with model group the difference in temperature at 120min of both herb group 1 and 2 had significant differentia( P <0.01) ; compare between these two herb groups also had significant differentia( P <0. 01). This data indicates that chuanhuning injection can magnificently inhibit the yeast inducing fever, and the effect is correlated with dosage.2. changes of PGE2 and cAMP content in hypothalamus of fevered rats treated, with chuanhuningCompared with control group the PGE2 and cAMP content in hypothalamus of model group is more higher, and difference of mean value has significant val-ue(P <0.01) ; The herb can efficiently inhibit the upraise of PGE2 and cAMP content in hypothalamus tissues, compared with model group (P<0.01,P<0. 01). Difference between these two herb groups also has statistical significance ( P < 0. 05 ). The content of PGE2 and cAMP in hypothalamus is positively correlative with the difference in temperature at 360min in all these groups (P <0. 01,P<0.01).Conclusion1. Our test has proved that chuanhuning has significant antipyretic effect on fevered rats induced by dry - yeast and this effect has dosage independence.
|
PDF Full Text Request