GST-π And Muc1 Gene Expression In Ovarian Carcinoma Tissue And Their Clinical Significance

Objective: The purposes of this study is to detect the expression of glutathione S-transferase pi(GST- ) and mucl gene in the patients with ovarian cancer, and to analysis their diagnostic sense and the value of combined examination of those two genes.Method: The expression of GST- and mucl genes in ovarian carcinoma tissue and normal tissue from 26 patients and 5 benign epithelial ovarian neoplasm were examined by qualitative reverse transcription polymerase chain reaction(RT-PCR).Results: The positive rate of GST- and mucl gene expression in ovarian cancer tissue were 73.1% and 80.8% respectively. Their expression in ovarian cancer tissue were significantly higher than that (7.7% and 19.2%) in the normal ovarian tissue(p<0.01). The total positive rate of GST- and mucl was 92.3% in carcinoma samples. There was a increasing tendency compared with single gene expression rate. We also found there were significant increasing of co-expression rate of GST- and mucl genes in ovarian carcinoma tissue versus normal ovarian tissue (61.5% versus 0, p<0.01). The expression of GST- and mucl genes had no significant correlation with tumor stage, histological types and pathology grade (p>0.05).Conclusion: Both GST-Ï€ and mud can be used for early clinical diagnosis of ovarian cancer. Combined examination of those two genes may have more clinical significance.