| The small bowel is the largest organ of lymphocyte in the body and the small bowel transplantation (SBT) is the exclusive assured method of curing the telophase function failure of small bowel. SBT can make the patients get rid of the worry of TPN and resume the natural life. But SBT will take place severe rejection inducing the failure of the operation because small bowel is the high immunocompetent bacterial organ. Following the immunosuppressant(for example: cyclosporine A and Tacrolimus) and multifarious strong drugs of anti-bacterium, anti-epiphyte and anti-virus used in clinic in the past several years, various syndrome was decreased after SBT and the long-term livability of the transplanted small bowel has been increased obviously. At present, it has taken a lot of basic study on SBT including immunity, infection, reperfusion injury and so on, and ithas obtained much progress. First, we built the model of the segmental SBT and the model of heat ischemia reperfusion of SBT in rats, then mensurated the serum level of nitric oxide(NO) , the activity of nitric oxide synthase (NOS) , the activity of inducible nitric oxide synthase (iNOS) and the changing of hypoxia induced factor 1 a(HIF-la)of small bowel tissue. We studied the relationship of NO, NOS, iNOS and HIF-la with acute graft rejection and offered a new method for the clinical therapy of small bowel transplantation. AIMl.To master the technic to build the model of the segmental small bowel transplantation in rats.2. To study the relationship of the serum level changing of nitric oxide with acute graft rejection after small bowel transplantation in rats.3. To study the changing of the activity of nitric oxide synthase and inducible nitric oxide synthase and expression of hypoxia induced factor 1 a and to discuss the effect and correlative function of hypoxia induced factor 1 a in small bowel transplantation of rats.METHODThe experiment was shared into the following groups: group A (control group, the common SD rats);group B(heat ischemia group) :to build the model of heat ischemia of rats, nipped the superior mesentery artery for 1 hour and shared into the following subgroups according to reperfusion:0 hour,1 hour,2 hour,6 hour; group C (cold ischemiagroup):cold ischemia was performed for 2.5 hour; group D (isograft group):To build the model of the segmental SBT in rats(keeping cold ischemia for 2.5 hour),to supply routine fluid replacement and antibiotic after the operation; group E(allograft group): To build the model of the segmental SBT in rats(keeping cold ischemia for 2.5 hour), to supply routine fluid replacement and antibiotic after the operation as well as group D; group F: To build the model of the segmental SBT in rats (keeping cold ischemia for 2. 5 hour), to perform allograft, to supply routine fluid replacement and antibiotic and cyclosporine A by injecting muscle after the operation. D, E and F groups were taken blood and small bowel tissue in the 3rd day, 5th day and 7th day after operation, other groups were taken blood and small bowel tissue according to the time points of themselves respectively. The specimen of small bowel tissue was stained with hematoxylin and eosin for pathological examination and we observed the changing of histopathology. The serum NO level were mensurated by the method of nitric acid deoxidization synthase. The activity of NOS and iNOS of small bowel tissue were mensurated by spectrophotometer. The expression of HIF-1a were mensurated by the method of immunohistochemistry. RESULTS1.The pathological examination of grafts from group E showed that the degree of acute rejection was from light to weight in the 3rd day,5th day and 7th day in turn; the fraction of grafts from group F showed light degree of acuterejection in the 5th day and 7th day after SBT; group D had not pathological evidence of acute rejection.2. The serum NO level of group B showed the degressive direction from 0 hour ,and the serum NO level of 0 hour subgroup and 1 hour subgroup were significant different from group A(P<... |
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