Antitumor Efficacy Of The Specific Cytotoxic T Lymphocytes Activated By Dendritic Cells To Glioma In Vitro

Objective To investigate the antitumor efficiency of the special cytotoxic T lymphocytes activated by dendritic cells loaded with glioma antigens in vitro and the feasibility of immunotherapy of glioma with dendritic cells for clinical application. Method Glioma cells were prepared by primary culture in vitro and tumour antigens were extracted by thawing and freezing. Peripheral blood monocytes were cultured to produce DCs with recombinant human granulocyte-macrophage colony - stimulating factor, recombinant human interleukin-4, recombinant human tumor necrosis factor- . After pulsed in vitro with glioma lysates, DCs were used to induce T lymphocytes into cytotoxic T lymphocytes for glioma cells. The cytotoxicity of CTLs to patient' s glioma cells was assayed by MTT. Result After culter for two days, cells began to increase in size and be charactered with dendritic morphology. After 10 days, more CDla , CD80, CD83, CD86, HLA-DR were detected by flow cytometer. Dendritic cells stimulated lymphocytes to increase greatly in Allo-MLRs. It was obvious when the proportion was 1 to 10. The CTLs could kill glioma cells markedly in vitro and the killing rate of glioma cells was more higher than that of K562 (P<0.01) .The ratio increased with the improvement of the proportion of effector cells to target cells. The CTLs were more effective to glioma than the CTLs cultured without glioma antigens (P<0.01) . Tow kinds of CTLs had similar effect to K562 (P>.05) .Conclusion The antigen specific CTLs killed glioma cells effectively and specifically, which might play a great role in clinical therapy.