Establishment Of Transgenic Mouse To Express HBV Binding Protein And Human Annexin V

HBV is able to infect only humans and a few kinds of primates, which impeded a more thorough understanding of HBV and HBV infection disease. The absence of specific cellular receptor(s) constitutes the barrier of HBV infection and replication in nonhuman hepatocytes. According to recent research advance in HBV receptors, transfected HepG2 and CHO cells by HBV binding protein cDNA acquired a remarkable virus binding capability . Similarly, transfected rat FTO 2B cells by human Annexin VcDNA. We try to get a HBV infection mouse model by establishing transgenic mice to express HBV binding protein and human Annexin V by transgenic animal technology ,by which animal can express foreign gene to get new character. We get mouse albumin enhancer and promoter from mouse genome by PCR and HBV binding protein cDNA, Annexin VcDNA from RNA of HepG2 cells by RT-PCR. The two receptor genes are linked by IRES(internal ribosomal entry site) under the mouse albumin enhancer and promoter. Five transgenic founder mice are generated by microinjecting. They are characterized by PCR and Southern Blot Hybridization. One of them express the transgene on the surface of Hepatocytes by FACS analysis. The transgenic mice were inoculated HBV in vivo, but until now, we have no evidence that HBV binding protein and human Annexin V transgenic mouse are able to be infected by HBV.