The Anti-hepatic Fibrosis Effects Of BJ-JN And Its Mechanism Of Action

To explore the effect of BJ-JN on liver injury and liver fibrosis, several experimental liver injury models were adopted. The protective mechanism of BJ-JN (3, 6, 12 g. kg-1 d-1) on hepatic damage was analyzed preliminarily. The method of serum pharmacology was used to observe the effect of drug-contained serum of BJ-JN on HSC-T6 cell line in vitro. The main results are as follows.1. Effect of BJ-JN on D-GalN induced acute liver injury in miceThe liver index and the serum content of ALT induced by D-GalN were significantly reduced by high dose of BJ-JN. Three dosage of BJ-JN alleviated the degree of hepatocytes injury induced by D-GalN in pathological examination.2. Effect of BJ-JN in CCl4 induced chronic liver injury in ratsThe elevation of serum ALT, AST, MDA and NO level were significantly reduced and the reduction of liver T-SOD content, serum A/G and thymus index was increased by BJ-JN. Three dosage of BJ-JN obviously alleviated the degree of liver fibrosis induced by CCl4. Not only the elevation of HA level in serum was reduced, but also the severity of liver fibrosis was significantly relieved in pathological examination, especially in rats injured by CCl4 for 19 weeks. The proliferation and collagen secreting of HSC isolated from liver fibrotic rats were remarkably inhibited by high-dose BJ-JN treatment. The results showed that BJ-JN has obviously anti-fibrosis effect and the mechanism of action might be related to its anti-oxidative activity, immune regulation and the inhibitory effect on HSC function.3. Effect of BJ-JN on BCG+LPS induced immunologyical liver injury in miceThe elevation of serum ALT content was obviously reduced by three dosage of BJ-JN. It was also found that BJ-JN could restore the diminished splenocyte proliferation induced by ConA and reduced IL-1 and TNF-a production by peritoneal macrophages(M ) in BCG + LPS injured mice. The high-dose BJ-JN obviously alleviated the degree of hepatocytes injury and the infiltration of inflammatory cell induced by BCG+LPS. BJ-JN remarkably promoted hepatocytes regeneration in pathological examination. These results showed that BJ-JN not only has the protective effective, but also has anti-inflammatory and immunoregulatory effects on immunologyical liver injury in mice.4. The serum pharmacology study of BJ-JNThe results showed that the drug-contained serum of BJ-JN (12 g. kg-1) significantlyrestrained the proliferation and collagen secreting of HSC-T6 cell in vitro.To sum up, BJ-JN has the protective effects on three experimental models (acute, chronic and immunological liver injury). The results in vivo and in vitro suggested that its mechanism of action might be associated with the anti-oxidative activity, immunoregulation and anti-inflammatory effect as well as the inhibitory effect on the function of HSC.