Study On Biological Characteristics Of BNX Mice And Its Animal Tumor Model

This article sum up the biological features of T, B, NK cell immunodeficient mouse-BNX mouse. In order to provide primitive data for BNX mice to use in the future we also explored the replication of BNX mice in tumor animal model of human gastric tumor (MKN-45 )and SV40T transgenic prostate tumor model.This study on some biological characteristics of BNX mice was made through periodically measuring the weight , analyzing breeding character, graphing growth curve , examining hematological and blood biochemical parameters. The result discovering that BNX was an severe immunodeficient mice;Its breeding performance was high: its verage litter number was 8.2?.6 (4.0 homozygous mice) and survival rate was 99.3% Breeding performance was higher than their background strain C57BL/6 mouse slightly. Weight of BNX mice increased before 18 week, it was relatively slow to keep in the platform for body to increase weight later stage. Male mice increased weight quickly than female mice, their weight were 29.2?.2g and 25.4?.8g respectively. Biochemical indexes and blood physiology related to animal's immune function, such as content of lymphocyte , total albumen and content of albumin ,etc of BNX mice was less than nude mice remarkable extremely, content of lymphocyte lower by 40% than the nude mice, the total amount of leucocytes and content of lymphocyte of BNX mice was (3.37?.69)109 / L , 26.4% respectively, it only reaches 1/3 of the normal mice. All of it verified that BNX mice was a immunodeficient mice.To established the high metastasis tumor animal's model of gastric tumor model(MKN-45) ,we used orthotopic transplant and subcutaneous transplant. The parameters of tumor formation rate, metastasis rate and so on of BNX mice was superior to nude mice and SCID mice in subcutaneous transplant tumor model, except the growth speed of tumor, especially in metastasis rate of tumor, BNX mice tumor metastasis rate of lung reached 100% , and liver metastasis rate was 89%, it had better target tropism tumor metastasis rate than nude mice and SCID mice; In orthotopictransplant tumor animal's model of BNX mice, tumor formation rate was 95.5% and its tumor metastasis rate of lung liver -. lymph midriff and formation rate of ascites was 100.0?K 76.2% 90.5% > 28.6% and 57.1% respectively, higher than contrast group of nude mice notably. The result showed that orthotopic transplant tumor animal model of BNX mice had reproduced the clinical course of people's gastric tumor basically, and showed that the replication of BNX mice in human gastric tumor MKN-45 tumor animal model especially in metastasis tumor animal model was better than nude mice and SCID mice.Though SV40T transgenic prostate tumor animal's model of FVB mice could offer the ideal animal model for learning to research the pathogeny of disease of the prostate tumor and cure it. But its reproductive capacity was low being occur disease early, and limited its popularizing and application. We tried to transplant it to their background strain mice (FVB mice) with subcutaneous transplant, but had not succeeded. To explore and establish SV40T transgenic prostate tumor animal's model, this research used BNX mice through subcutaneous transplant succeeding in solving the problems of preserving and application of SV40T transgenic prostate tumor. We also transplant it to his background strain mice(FVB mice) and nude mice. By experimental observation, it tumor formation time was 11.5?. 6 days, average tumor-bearing of BNX mice wad 66?.7 days and its tumor diameter could reach 6.0 centimetre, tumor formation rate was 100.0%. Measure by PCR, subcutaneous tumo liver and lung were found SV40T gene, indicate that this tumor come from SV40T transgenic FVB mice and the metastasis of liver and lung happened, pathology verifies that the liver had the tumor. Because PCR method sensitivity high, this model is ideal tumor animal model for study tumor micrometastasis theory...